Characterization of human Sec16B: indications of specialized, non-redundant functions.
Characterization of human Sec16B: indications of specialized, non-redundant functions.
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DOI：
10.1038/srep00077
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发表时间：
2011
影响因子：
4.6
通讯作者：
Stephens, David J.
中科院分区：
文献类型：
--
作者：
Budnik, Annika;Heesom, Kate J.;Stephens, David J.
内质网(ER)代表着进入分泌途径的入口点，新合成的蛋白质和脂类从这里被输送到高尔基体。内质网的选择性货物出口是通过COPII在内质网特定位置的组装来实现的，即所谓的过渡性内质网(TER)。最早在酵母中发现的外周膜蛋白Sec16定位于过渡性内质网，在这些部位的组织中起着关键作用。Sec16定义了TER，并被认为是COPII涂层组件的脚手架。在人类中存在两种Sec16亚型，较大的Sec16A和较小的Sec16B。然而，这两种异构体之间的功能差异是模糊的。在这里，我们描述了Sec16B相对于Sec16A的局部化和动力学特征，提供了Sec16B可能是Sec16的一种轻微形式或特殊形式的证据，并且它与Sec16A在功能上不是多余的。
The endoplasmic reticulum (ER) represents the entry point into the secretory pathway and from here newly synthesized proteins and lipids are delivered to the Golgi. The selective cargo export from the ER is mediated by COPII-assembly at specific sites of the ER, the so-called transitional ER (tER). The peripheral membrane protein Sec16, first identified in yeast, localizes to transitional ER and plays a key role in organization of these sites. Sec16 defines the tER and is thought to act as a scaffold for the COPII coat assembly. In humans two isoforms of Sec16 are present, the larger Sec16A and the smaller Sec16B. Nevertheless, the functional differences between the two isoforms are ill-defined. Here we describe characterization of the localization and dynamics of Sec16B relative to Sec16A, provide evidence that Sec16B is likely a minor or perhaps specialized form of Sec16, and that it is not functionally redundant with Sec16A.
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影响因子：
4
作者：
Townley, Anna K.;Feng, Yi;Stephens, David J.
通讯作者：
Stephens, David J.
DOI：
10.1111/j.1600-0854.2006.00493.x
发表时间：
2006-12
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Watson P;Townley AK;Koka P;Palmer KJ;Stephens DJ
通讯作者：
Stephens DJ
影响因子：
3.3
作者：
Hammond, AT;Glick, BS
通讯作者：
Glick, BS
影响因子：
9.2
作者：
Connerly, PL;Esaki, M;Glick, BS
通讯作者：
Glick, BS
影响因子：
3.3
作者：
Gimeno, RE;Espenshade, P;Kaiser, CA
通讯作者：
Kaiser, CA