Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster.
Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster.
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DOI：
10.1038/srep32346
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发表时间：
2016-08-30
影响因子：
4.6
通讯作者：
Šimek P
中科院分区：
文献类型：
--
作者：
Koštál V;Korbelová J;Štětina T;Poupardin R;Colinet H;Zahradníčková H;Opekarová I;Moos M;Šimek P
建议用于果蝇（Drosophila melanogaster）胚胎的冷冻保存技术尚未准备好用于实际应用。长期保存D.黑腹菌菌株虽然急需，但并不存在。在此，我们描述了一个狭窄的低温区间下，D. melanogaster可以在静止状态下储存长达两个月。波动温度条件下，幼虫的发育停滞在游走前阶段，同时减少了间接冷害的积累。我们的生理学，代谢组学和转录组学分析表明，相比于在恒定低温下储存的幼虫，在FTR条件下储存的幼虫能够降低能量底物的消耗速率，利用FTR的短暂温暖事件来稳态控制代谢物水平，并且更有效地保护免受氧化损伤。
The cryopreservation techniques proposed for embryos of the fruit fly Drosophila melanogaster are not yet ready for practical use. Alternative methods for long-term storage of D. melanogaster strains, although urgently needed, do not exist. Herein, we describe a narrow interval of low temperatures under which the larvae of D. melanogaster can be stored in quiescence for up to two months. The development of larvae was arrested at the pre-wandering stage under fluctuating thermal regime (FTR), which simultaneously resulted in diminishing the accumulation of indirect chill injuries. Our physiological, metabolomic, and transcriptomic analyses revealed that compared to larvae stored at constant low temperatures, the larvae stored under FTR conditions were able to decrease the rates of depletion of energy substrates, exploited brief warm episodes of FTR for homeostatic control of metabolite levels, and more efficiently exerted protection against oxidative damage.
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影响因子：
3.7
作者：
Koštál V;Korbelová J;Rozsypal J;Zahradníčková H;Cimlová J;Tomčala A;Šimek P
通讯作者：
Šimek P
影响因子：
3.2
作者：
KOCOUREK, J;BALLOU, CE
通讯作者：
BALLOU, CE
影响因子：
1.9
作者：
Dorffling, K;Dorffling, H;Jurgens, HU
通讯作者：
Jurgens, HU
影响因子：
1.3
作者：
Denlinger, David L.
通讯作者：
Denlinger, David L.
影响因子：
3.4
作者：
ARAKAWA, T;TIMASHEFF, SN
通讯作者：
TIMASHEFF, SN