Molecular characterization of a Fus3/Kss1 type MAPK from Puccinia striiformis f. sp. tritici, PsMAPK1.
Molecular characterization of a Fus3/Kss1 type MAPK from Puccinia striiformis f. sp. tritici, PsMAPK1.
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DOI：
10.1371/journal.pone.0021895
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Guo J;Dai X;Xu JR;Wang Y;Bai P;Liu F;Duan Y;Zhang H;Huang L;Kang Z
条锈菌小麦条锈菌（Pst）是一种专性生体营养型真菌，在世界范围内引起小麦条锈病。由于缺乏可靠的转化和基因破坏方法，对Pst基因在致病过程中的功能了解有限。丝裂原活化蛋白激酶（MAPK）基因在植物病原真菌的侵染过程中起着重要的调控作用。在本研究中，我们确定了第一个MAPK基因PsMAPK 1在Pst。系统进化分析表明PsMAPK 1是一个YERK 1 MAP激酶，属于Fus 3/Kss 1类。在6株Pst中检测到PsMAPK 1基因编码区的单核苷酸多态性（SNP）和插入/缺失。实时荧光定量RT-PCR分析表明，PsMAPK 1的表达诱导感染早期阶段和吸器形成期间达到高峰。当在禾谷镰刀菌中表达时，PsMAPK 1部分拯救了营养生长和致病性的map 1突变体。它也部分弥补了Magnaporthe pmk 1突变体在附着胞形成和植物感染方面的缺陷。这些结果表明F. graminearum和M. PsMAPK 1可作为Pst基因功能分析的替代系统，PsMAPK 1可能在Pst侵染植物和侵染生长的调控中发挥作用。
Puccinia striiformis f. sp. tritici (Pst) is an obligate biotrophic fungus that causes the destructive wheat stripe rust disease worldwide. Due to the lack of reliable transformation and gene disruption method, knowledge about the function of Pst genes involved in pathogenesis is limited. Mitogen-activated protein kinase (MAPK) genes have been shown in a number of plant pathogenic fungi to play critical roles in regulating various infection processes. In the present study, we identified and characterized the first MAPK gene PsMAPK1 in Pst. Phylogenetic analysis indicated that PsMAPK1 is a YERK1 MAP kinase belonging to the Fus3/Kss1 class. Single nucleotide polymerphisms (SNPs) and insertion/deletion were detected in the coding region of PsMAPK1 among six Pst isolates. Real-time RT-PCR analyses revealed that PsMAPK1 expression was induced at early infection stages and peaked during haustorium formation. When expressed in Fusarium graminearum, PsMAPK1 partially rescued the map1 mutant in vegetative growth and pathogenicity. It also partially complemented the defects of the Magnaporthe oryzae pmk1 mutant in appressorium formation and plant infection. These results suggest that F. graminearum and M. oryzae can be used as surrogate systems for functional analysis of well-conserved Pst genes and PsMAPK1 may play a role in the regulation of plant penetration and infectious growth in Pst.
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影响因子：
64.5
作者：
Good M;Tang G;Singleton J;Reményi A;Lim WA
通讯作者：
Lim WA
通过大米爆炸真菌中的不同机制，可以感觉到多个植物表面信号以形成appressorium。
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作者：
Mehrabi, Rahim;Ding, Shengli;Xu, Jin-Rong
通讯作者：
Xu, Jin-Rong