New cross-linking quinoline and quinolone derivatives for sensitive fluorescent labeling.
New cross-linking quinoline and quinolone derivatives for sensitive fluorescent labeling.
复制标题
DOI：
10.1007/s10895-012-1039-z
复制
发表时间：
2012-07
影响因子：
2.7
通讯作者：
Mustaev, Arkady
中科院分区：
文献类型：
--
作者：
Pillai, Shyamala;Kozlov, Maxim;Marras, Salvatore A. E.;Krasnoperov, Lev N.;Mustaev, Arkady
A variety of contemporary analytical platforms, utilized in technical and biological applications, take advantage of labeling the objects of interest with fluorescent tracers—compounds that can be easily and sensitively detected. Here we describe the synthesis of new fluorescent quinoline and quinolone compounds, whose light emission can be conveniently tuned by simple structural modifications. Some of these compounds can be used as sensitizers for lanthanide emission in design of highly sensitive luminescent probes. In addition, we also describe simple efficient derivatization reactions that allow introduction of amine- or click-reactive cross-linking groups into the fluorophores. The reactivity of synthesized compounds was confirmed in reactions with low molecular weight nucleophiles, or alkynes, as well as with click-reactive DNA-oligonucleotide containing synthetically introduced alkyne groups. These reactive derivatives can be used for covalent attachment of the fluorophores to various biomolecules of interest including nucleic acids, proteins, living cells and small cellular metabolites. Obtained compounds are characterized using NMR, steady-state fluorescence spectroscopy as well as UV absorption spectroscopy. The online version of this article (doi:10.1007/s10895-012-1039-z) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
4.7
作者：
Krasnoperov LN;Marras SA;Kozlov M;Wirpsza L;Mustaev A
通讯作者：
Mustaev A
影响因子：
3.6
作者：
BISSELL, ER;MITCHELL, AR;SMITH, RE
通讯作者：
SMITH, RE
影响因子：
3.6
作者：
LEWIS, FD;REDDY, GD;KOJIMA, M
通讯作者：
KOJIMA, M
DOI：
10.1016/s1010-6030(00)00280-x
发表时间：
2000-06-27
影响因子：
4.3
作者：
Chen, JY;Selvin, PR
通讯作者：
Selvin, PR
影响因子：
1.8
作者：
Uray, G;Niederreiter, KS;Fabian, WMF
通讯作者：
Fabian, WMF