Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.
Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.
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DOI：
10.1080/19396368.2022.2073292
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发表时间：
2022-10
影响因子：
2.4
通讯作者：
Murphy, Susan K.
中科院分区：
文献类型：
--
作者：
Schrott, Rose;Greeson, Katherine W.;King, Dillon;Crow, Krista M. Symosko;Easley, Charles A.;Murphy, Susan K.
大麻在美国的使用正在增加，男性在生育高峰期的消费量最高。我们先前证明了人类和大鼠暴露于大麻后精子DNA甲基化的广泛变化，包括在神经发育中重要的基因。在这里，我们使用体外人类精子发生模型来概括慢性大麻使用，并评估精原干细胞（SSC）和精子样细胞中印迹和自闭症谱系障碍（ASD）候选基因的DNA甲基化。母源印记基因SGCE和GRB 10的甲基化在SSC和精子样细胞中分别发生了显著改变，而PEG 3在精子样细胞中的甲基化差异显著。10个随机选择的ASD候选基因中的两个，HCN 1和NR4A2，在SSC样细胞中暴露于大麻后甲基化发生了显着改变。这些结果支持了我们在人类队列中的发现，并提供了一种新的工具，用于获得对父亲使用大麻与后代ASD风险之间关系的机械见解。
Cannabis use in the United States is increasing, with highest consumption among men at their peak reproductive years. We previously demonstrated widespread changes in sperm DNA methylation with cannabis exposure in humans and rats, including genes important in neurodevelopment. Here, we use an in vitro human spermatogenesis model to recapitulate chronic cannabis use and assess DNA methylation at imprinted and autism spectrum disorder (ASD) candidate genes in spermatogonial stem cell (SSC)- and spermatid-like cells. Methylation at maternally imprinted genes SGCE and GRB10 was significantly altered in SSC- and spermatid-like cells, respectively, while PEG3 was significantly differentially methylated in spermatid-like cells. Two of ten randomly selected ASD candidate genes, HCN1 and NR4A2, had significantly altered methylation with cannabis exposure in SSC-like cells. These results support our findings in human cohorts and provide a new tool with which to gain mechanistic insights into the association between paternal cannabis use and risk of ASD in offspring.
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DOI：
10.1038/npp.2017.198
发表时间：
2018-01
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
Hasin DS
通讯作者：
Hasin DS
影响因子：
64.5
作者：
Gkountela S;Zhang KX;Shafiq TA;Liao WW;Hargan-Calvopiña J;Chen PY;Clark AT
通讯作者：
Clark AT
DOI：
10.1073/pnas.1532175100
发表时间：
2003-07-08
影响因子：
11.1
作者：
Charalambous, M;Smith, FM;Ward, A
通讯作者：
Ward, A
人类原始生殖细胞的转录组和 DNA 甲基化组景观
影响因子：
64.5
作者：
Guo, Fan;Yan, Liying;Qiao, Jie
通讯作者：
Qiao, Jie
影响因子：
3.5
作者：
Huang JM;Kim J
通讯作者：
Kim J