Molecular characterization of a defensin gene from a hard tick, Dermacentor silvarum.
Molecular characterization of a defensin gene from a hard tick, Dermacentor silvarum.
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硬蜱 Dermacentor silvarum 防御素基因的分子特征。
DOI：
10.1186/s13071-014-0625-0
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发表时间：
2015-01-15
影响因子：
3.2
通讯作者：
Dai J
中科院分区：
文献类型：
--
作者：
Wang J;Bian G;Pan W;Feng T;Dai J
背景：扁虱广泛分布于世界各地，被认为是多种人类疾病的媒介。硬蜱防御素是一类抗菌肽家族，是抵御病原体的第一道防御素。发现：从中国东北地区采集的森林硬蜱的cdna文库中，发现了一个类似防御素的基因，命名为DS-Defensin。DS-防御素基因全长225bp，编码74个氨基酸。DS-防御素的核苷酸序列与推测的边缘尘螨防御素的同源性为98.2%。RT-PCR结果表明DS-防御素在硬蜱唾液腺和中肠中广泛表达，其中中肠表达水平较高。DS-防御素对多种革兰氏阳性和革兰氏阴性细菌以及真菌白色念珠菌具有广泛的抗菌活性。结论：我们鉴定了中国森林假丝酵母菌的一种功能性防御素。DS-防御素对多种革兰氏阳性菌和革兰氏阴性菌具有杀菌活性。DS-防御素作为一种具有抗菌活性的新分子有望引入医学领域。
Background:Ticks are distributed worldwide and considered as vectors of many human diseases. Tick defensins, a family of antimicrobial peptides, form the first line of defense against pathogens.Findings:A defensin-like gene, named Ds-defensin, was identified from a cDNA library of the hard tick Dermacentor silvarum collected from northeast China. The full-length cDNA of Ds-defensin was 225 bp, encoding a 74 amino acid peptide. The nucleotide sequence of Ds-defensin shared 98.2% similarity to putative defensin from Dermacentor marginatus. RT-PCR results suggested that Ds-defensin was extensively expressed in tick salivary gland and midgut, with a higher expression level in midgut. Ds-defensin showed broad antimicrobial activity against various Gram-positive and Gram-negative bacteria, as well as the fungus Candida albicans.Conclusions:We characterized a functional defensin from D. silvarum of China. Ds-defensin showed bactericidal activity against various Gram-positive and Gram-negative bacteria. Ds-defensin can be expected to be introduced to the medical field as a new molecule with antibacterial activity.
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影响因子：
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