Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum).
Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum).
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碱溶性果胶是豌豆 (Pisum sativum) 根际细胞中铝固定化的主要靶标
DOI：
10.3389/fpls.2016.01297
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yu M
中科院分区：
文献类型：
--
作者：
Yang J;Qu M;Fang J;Shen RF;Feng YM;Liu JY;Bian JF;Wu LS;He YM;Yu M
本研究探讨了豌豆根缘细胞(RbCs)铝离子迁移率与细胞壁中铝结合量的差异这一假说，从而为根尖和RbCs在铝胁迫下提供保护。豌豆(P.sativum L.‘中晚6号’)在雾化培养条件下进行铝处理。红细胞中铝的浓度远高于根尖。一个根尖周围的红细胞(104个红细胞)中的铝含量约占根尖(0-2.5 mm)总铝含量的24.5%，表明红细胞对铝毒害下的根尖具有屏蔽作用。细胞壁分析表明，铝主要积累在红细胞的碱溶果胶(果胶2)中。这可能是由于铝毒性下糖醛酸的显著增加，果胶2对铝的吸附能力[是螯合可溶性果胶(果胶1)的5.3倍]，以及果胶2的铝脱附率(8.5%)低于果胶1(68.5%)。这些结果表明，果胶2是豌豆红细胞铝固定的主要靶点，它影响铝进入红细胞胞内间隙和向根尖的迁移，从而保护根尖和红细胞膜免受铝毒害。
We investigated the hypothesis that a discrepancy of Al binding in cell wall constituents determines Al mobility in root border cells (RBCs) of pea (Pisum sativum), which provides protection for RBCs and root apices under Al toxicity. Plants of pea (P. sativum L. ‘Zhongwan no. 6’) were subjected to Al treatments under mist culture. The concentration of Al in RBCs was much higher than that in the root apex. The Al content in RBCs surrounding one root apex (104 RBCs) was approximately 24.5% of the total Al in the root apex (0–2.5 mm), indicating a shielding role of RBCs for the root apex under Al toxicity. Cell wall analysis showed that Al accumulated predominantly in alkali-soluble pectin (pectin 2) of RBCs. This could be attributed to a significant increase of uronic acids under Al toxicity, higher capacity of Al adsorption in pectin 2 [5.3-fold higher than that of chelate-soluble pectin (pectin 1)], and lower ratio of Al desorption from pectin 2 (8.5%) compared with pectin 1 (68.5%). These results indicate that pectin 2 is the primary target of Al immobilization in RBCs of pea, which impairs Al access to the intracellular space of RBCs and mobility to root apices, and therefore protects root apices and RBCs from Al toxicity.
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影响因子：
4.9
作者：
VONUEXKULL, HR;MUTERT, E
通讯作者：
MUTERT, E
影响因子：
4.9
作者：
Kopittke, P. M.;McKenna, B. A.;Menzies, N. W.
通讯作者：
Menzies, N. W.
影响因子：
6.4
作者：
Rangel, Andres Felipe;Rao, Idupulapati Madhusudana;Horst, Walter Johannes
通讯作者：
Horst, Walter Johannes
影响因子：
6.2
作者：
Braccini, I;Pérez, S
通讯作者：
Pérez, S
影响因子：
7.3
作者：
Chang, YC;Yamamoto, Y;Matsumoto, H
通讯作者：
Matsumoto, H