Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract.
Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract.
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DOI：
10.3390/ijms22179305
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发表时间：
2021-08-27
影响因子：
5.6
通讯作者：
Bulgakov V
中科院分区：
文献类型：
--
作者：
Shkryl Y;Rusapetova T;Yugay Y;Egorova A;Silant'ev V;Grigorchuk V;Karabtsov A;Timofeeva Y;Vasyutkina E;Kudinova O;Ivanov V;Kumeiko V;Bulgakov V
本研究报告了一种绿色化学方法，用于快速和容易的生物合成银（Ag），金（Au），和银/Au纳米粒子使用紫草愈伤组织提取物作为还原剂和封端剂。采用紫外-可见光谱、X射线衍射和透射电子显微镜对纳米粒子进行了表征。我们的结果表明，形成的晶体金属纳米结构的球形和非球形形状。能量色散X射线（EDX）光谱显示出在银和金区域中的特征峰，证实了在monoclonal颗粒中存在相应的元素以及在monoclonal颗粒中存在两种元素。傅里叶变换红外光谱（FTIR）证实了多糖和多酚的作用。刺桐提取物作为金属离子的主要还原剂和封端剂。此外，我们的研究结果表明，多糖样品和含有次级代谢产物的组分从L。刺桐都能够产生大量的金属纳米颗粒。生物合成的纳米粒子表现出对小鼠神经母细胞瘤和胚胎成纤维细胞的细胞毒性，这是相当高的银纳米粒子和银/Au纳米粒子含有较高的摩尔比的银。然而，成纤维细胞迁移没有受到任何测试的纳米颗粒的显著影响。所获得的结果为金属纳米颗粒的安全生物生产提供了一个新的例子，但需要进一步的研究来揭示其毒性机制，以便评估其生物医学效力。
The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), and bimetallic Ag/Au nanoparticles using the callus extract of Lithospermum erythrorhizon as a reducing and capping agent. The biosynthesized nanoparticles were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). Our results showed the formation of crystalline metal nanostructures of both spherical and non-spherical shape. Energy dispersive X-ray (EDX) spectroscopy showed the characteristic peaks in the silver and gold regions, confirming the presence of the corresponding elements in the monometallic particles and both elements in the bimetallic particles. Fourier-transform infrared (FTIR) spectroscopy affirmed the role of polysaccharides and polyphenols of the L. erythrorhizon extract as the major reducing and capping agents for metal ions. In addition, our results showed that the polysaccharide sample and the fraction containing secondary metabolites isolated from L. erythrorhizon were both able to produce large amounts of metallic nanoparticles. The biosynthesized nanoparticles demonstrated cytotoxicity against mouse neuroblastoma and embryonic fibroblast cells, which was considerably higher for Ag nanoparticles and for bimetallic Ag/Au nanoparticles containing a higher molar ratio of silver. However, fibroblast migration was not significantly affected by any of the nanoparticles tested. The obtained results provide a new example of the safe biological production of metallic nanoparticles, but further study is required to uncover the mechanism of their toxicity so that the biomedical potency can be assessed.
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影响因子：
5.6
作者：
Amin M;Anwar F;Janjua MRSA;Iqbal MA;Rashid U
通讯作者：
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