Identification of phosphocholine plasmalogen as a lipid component in mammalian pulmonary surfactant using high-resolution 31P NMR spectroscopy.
Identification of phosphocholine plasmalogen as a lipid component in mammalian pulmonary surfactant using high-resolution 31P NMR spectroscopy.
复制标题
使用高分辨率 31P NMR 光谱鉴定磷酸胆碱缩醛磷脂作为哺乳动物肺表面活性剂中的脂质成分。
DOI：
10.1021/bi00052a005
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Dluhy,RA
中科院分区：
文献类型：
--
作者：
Rana,FR;Harwood,JS;Mautone,AJ;Dluhy,RA
摘要：用高分辨率31P核磁共振波谱分析了两种不同来源的哺乳动物肺表面活性物质的磷脂组成。在相当窄的通常较宽的31P磷脂信号的条件下，这些表面活性物质的溶液核磁共振谱明确地表明，在哺乳动物肺表面活性物质中，磷胆碱血浆蛋白原(即1-O-(1‘-alkenyl)-2-acyl-szt-glycero-3-phosphocholine))作为主要的次要成分(~4mol%)存在。从成年牛肺表面活性物质提取液和羊(羊)胎肺液中均可鉴定出磷胆碱(PC)纤溶酶原。PC血浆蛋白原此前尚未鉴定出任何哺乳动物肺表面活性物质制剂。这些制剂中PC纤溶酶原的含量与磷甘油(GB)的含量相当，后者以前被认为是肺表面活性物质中第二常见的磷脂类别。PC纤溶酶原在肺表面活性物质中的存在可能具有重要的生理意义，并立即为肺表面活性物质的生化和生物物理研究提供新的方向。肺表面活性物质是由肺泡II型上皮细胞合成和分泌的脂类(90wt%)、蛋白质(8wt%)和碳水化合物(2wt%)的复杂混合物。这种多相表面活性物质混合物的作用是将肺内空气-肺泡界面的表面张力降低到接近于零，从而为呼吸所需的支气管-肺泡单位提供必要的机械稳定性(Scarpelli，1988)。肺表面活性物质合成和释放不足是早产儿呼吸窘迫以及某些形式的成人呼吸窘迫综合征的原因。肺表面活性物质中85%以上的脂类是磷脂(King，1984)。到目前为止，磷胆碱和磷酸甘油(PC‘s和PC’s)1已被确定为哺乳动物肺表面活性物质中的主要磷脂类。PC约占肺表面活性物质重量的70%-85%，其中1，2-二棕榈酰甘油-3-磷酸胆碱(DPPC)占PC总成分的60%以上。含有PG及其头基的脂类最多可占肺表面活性物质磷脂的10%。其他磷脂类(PE、SM、PI、PS和CL)发生在相对较低的水平[<；2-5mol%(King，1984)]。至少有四种蛋白质被分离出来，分别命名为SP-A、SP-B、SP-C和SP-D。
Revised Manuscript Received November 10, 1992 abstract: High-resolution 31P NMR spectroscopy was used toanalyze the phospholipid composition of mammalian pulmonary surfactant from two different sources. Under conditions which considerably narrow the usually broad 31P phospholipid signals, solution-phase NMR spectra of these surfactant preparations unequivocally demonstrate that a phosphocholine plasmalogen (ie, 1-O-(1'-alkenyl)-2-acyl-szt-glycero-3-phosphocholine) exists as a major secondary component (~ 4 mol%) in mammalian pulmonary surfactant. Phosphocholine (PC) plasmalogen was identified in preparations obtained from both adult cow lung surfactant extract as well as in ovine (lamb) fetal pulmonary liquid. PC plasmalogenshave not previously been identified any mammalian pulmonary surfactant preparation. Theamount of PC plasmalogen in these preparations occurs at fractional levels that are comparable to that of phosphoglycerol (£), which previously had been thought of as the second-most common phospholipid class in pulmonary surfactant. Thepresence of PC plasmalogen in pulmonary surfactant may have important physiological ramifications and immediately suggests new directions for biochemical and biophysical investigations of pulmonary surfactant.Pulmonary surfactant is a complex mixtureof lipids (90 wt%), proteins (8 wt%), and carbohydrates (2 wt%) which is synthesized and secreted by alveolarepithelial type II cells. The function of this heterogeneous surfactant mixture is to reduce the surface tension at the air-alveolar interface inthe lungs to near zero, thereby providing the necessary mechanical stability to the broncho-alveolarunits required for breathing (Scarpelli, 1988). Inadequate synthesis and release of pulmonary surfactant is responsible for the respiratory distress experienced by premature infants as well as some forms of adult respiratory distress syndrome. Greater than 85% of the lipids found in pulmonary surfactant are phospholipids (King, 1984). To date, phosphocholines and phosphoglycerols (PC’s and PC’s) 1 have been identified as the primary phospholipid classes which are present inmammalian pulmonary surfactant. PC’s comprise roughly 70-85% of pulmonary surfactantby weight, with the specific PC species 1, 2-dipalmitoyl-in-glycero-3-phosphocholine (DPPC) making up more than 60% of the total PC component. The class of lipids containingPG and their headgroup may comprise up to 10% of the pulmonary surfactant phospholipids. The other phospholipid classes (PE, SM, PI, PS, and CL) occur at relatively low levels [< 2-5 mol%(King, 1984)]. At least four proteins, termed SP-A, SP-B, SP-C, and SP-D, have also been isolated from pulmonary
登录
查看更多内容
肺表面活性剂 SP-C 二级结构和取向的傅里叶变换红外研究及其对磷脂动态表面性质的影响。
影响因子：
2.9
作者：
Pastrana,B;Mautone,AJ;Mendelsohn,R
通讯作者：
Mendelsohn,R
成年兔肺表面活性物质分离物的物理、化学和生理特性。
影响因子：
2.1
作者：
K. Keough;E. Farrell;M. Cox;G. Harrell;H. Taeusch
通讯作者：
H. Taeusch
疏水性低分子量表面活性剂缔合多肽的大小和结构。
影响因子：
2.9
作者：
J. Johansson;T. Curstedt;B. Robertson;H. Jörnvall
通讯作者：
H. Jörnvall
肺表面活性剂载脂蛋白B/C对sn-2-[1-13C]二棕榈酰磷脂酰胆碱化学位移各向异性的影响。
影响因子：
3.4
作者：
A. Salmon;T. Wiedmann
通讯作者：
T. Wiedmann
兔肺表面活性材料的傅里叶变换红外和差示扫描量热研究。
DOI：
10.1016/0005-2736(87)90349-x
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Mautone,AJ;Reilly,KE;Mendelsohn,R
通讯作者：
Mendelsohn,R