Eyeblink tract tracing with two strains of herpes simplex virus 1.
Eyeblink tract tracing with two strains of herpes simplex virus 1.
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DOI：
10.1016/j.brainres.2022.148040
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发表时间：
2022-10-15
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
包括H129和McIntyre在内的神经侵袭性单纯疱疹-1（HSV-1）分离株在标记与感染细胞直接连接的高级神经元的突触处或附近交叉。H129主要在顺行方向传播，而McIntyre株仅在逆行方向传播。然而，尚不清楚一旦被H129或McIntyre的衍生物感染，神经元是否有功能。我们描述了一个以前未发表的HSV-1重组H129（HSV-373）表达mCherry荧光报告和一个新的麦金太尔重组（HSV-780）表达mCherry荧光团，并证明感染如何影响神经元的活力。每种重组病毒的行为相似，并在感染后4天传播到靶标。我们使用Fluoro-jade C测试了H129重组感染的神经元的神经变性，发现它们由于病毒感染而坏死。我们用HSV-772和HSV-780进行双重接种，以分别鉴定包括我们研究回路的顺行途径和逆行途径的细胞。我们研究了突触后标志物PSD-95的存在，它在突触可塑性中起作用，在HSV-772感染和双重感染大鼠（HSV-772和HSV-780）。PSD-95反应性降低HSV-772感染的神经元和双重感染的组织没有PSD-95反应性。这些新的重组病毒的感染追踪了感兴趣的回路，但是对包含该通路的细胞的功能研究是不可能的，因为病毒感染的神经元由于坏死而死亡，或者在病毒标记到达靶点时被剥夺了PSD-95。
Neuroinvasive herpes simplex-1 (HSV-1) isolates including H129 and McIntyre cross at or near synapses labeling higher-order neurons directly connected to infected cells. H129 spreads predominately in the anterograde direction while McIntyre strains spread only in the retrograde direction. However, it is unknown if neurons are functional once infected with derivatives of H129 or McIntyre. We describe a previously unpublished HSV-1 recombinant derived from H129 (HSV-373) expressing mCherry fluorescent reporters and one new McIntyre recombinant (HSV-780) expressing the mCherry fluorophore and demonstrate how infections affect neuron viability. Each recombinant virus behaved similarly and spread to the target 4 days post-infection. We tested H129 recombinant infected neurons for neurodegeneration using Fluoro-jade C and found them to be necrotic as a result of viral infection. We performed dual inoculations with both HSV-772 and HSV-780 to identify cells comprising both the anterograde pathway and the retrograde pathway, respectively, of our circuit of study. We examined the presence of postsynaptic marker PSD-95, which plays a role in synaptic plasticity, in HSV-772 infected and in dual-infected rats (HSV-772 and HSV-780). PSD-95 reactivity decreased in HSV-772-infected neurons and dual-infected tissue had no PSD-95 reactivity. Infection by these new recombinant viruses traced the circuit of interest but functional studies of the cells comprising the pathway were not possible because viral-infected neurons died as a result of necrosis or were stripped of PSD-95 by the time the viral labels reached the target.
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影响因子：
4.6
作者：
Broadhead MJ;Horrocks MH;Zhu F;Muresan L;Benavides-Piccione R;DeFelipe J;Fricker D;Kopanitsa MV;Duncan RR;Klenerman D;Komiyama NH;Lee SF;Grant SG
通讯作者：
Grant SG
影响因子：
6.1
作者：
Almeida, CG;Tampellini, D;Gouras, GK
通讯作者：
Gouras, GK
影响因子：
3.3
作者：
BARNETT, EM;CASSELL, MD;PERLMAN, S
通讯作者：
PERLMAN, S
DOI：
10.1007/978-1-60761-883-6_1
发表时间：
2011-01-01
期刊：
ANIMAL MODELS OF BEHAVIORAL ANALYSIS
影响因子：
--
作者：
Brown, Kevin L.;Woodruff-Pak, Diana S.
通讯作者：
Woodruff-Pak, Diana S.
影响因子：
1.5
作者：
Helfert, Stephanie M.
通讯作者：
Helfert, Stephanie M.