Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance.
Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance.
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南京菊TCP基因家族的特征及CnTCP4在耐寒性中的作用
DOI：
10.3390/plants11070936
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发表时间：
2022-03-30
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--
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植物特异性TCP转录因子在植物发育和逆境应答中起着关键作用。菊花比观赏多倍体菊花具有更强的耐寒性。然而，TCP基因家族是否在赋予C.南京人仍然不为人知。在此，我们在C. nankingense的TCPs，系统分析了它们与其他物种TCPs的系统发育关系和同线性，并对其低温表达谱进行了分析。系统发育分析表明，CnTCP蛋白可分为两类三个进化枝，具有典型的bHLH结构域。然而，C. nankingense和拟南芥中CnTCPs的蛋白结构和结合位点的分析表明，CnTCPs在C.南京人。此外，表达谱显示，大多数CnTCPs的表达在低温条件下下调，表明它们在植物对冷胁迫的反应中的重要性。值得注意的是，miR 319及其预测的靶基因CnTCP 2/4/14的表达导致对寒冷的快速反应。拟南芥CnTCP 4的过表达导致了对冷的敏感性，表明CnTCP 4可能在C.南京鹅掌楸对冷胁迫的反应我们的研究结果为菊花中该基因家族的功能基因组学研究奠定了基础。
Plant-specific TCP transcription factors play a key role in plant development and stress responses. Chrysanthemum nankingense shows higher cold tolerance than its ornamental polyploid counterpart. However, whether the TCP gene family plays a role in conferring cold tolerance upon C. nankingense remains unknown. Here, we identified 23 CnTCP genes in C. nankingense, systematically analyzed their phylogenetic relationships and synteny with TCPs from other species, and evaluated their expression profiles at low temperature. Phylogenetic analysis of the protein sequences suggested that CnTCP proteins fall into two classes and three clades, with a typical bHLH domain. However, differences between C. nankingense and Arabidopsis in predicted protein structure and binding sites suggested a unique function of CnTCPs in C. nankingense. Furthermore, expression profiles showed that expression of most CnTCPs were downregulated under cold conditions, suggesting their importance in plant responses to cold stress. Notably, expression of miR319 and of its predicted target genes, CnTCP2/4/14, led to fast responses to cold. Overexpression of Arabidopsis CnTCP4 led to hypersensitivity to cold, suggesting that CnTCP4 might play a negative role in C. nankingense responses to cold stress. Our results provide a foundation for future functional genomic studies on this gene family in chrysanthemum.
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