Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation.
Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation.
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DOI：
10.1016/j.cell.2009.04.044
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发表时间：
2009-05-15
期刊：
影响因子：
64.5
通讯作者：
Rudner DZ
中科院分区：
文献类型：
--
作者：
Sullivan NL;Marquis KA;Rudner DZ
复制染色体的组织和分离是所有生物体细胞分裂的重要组成部分。枯草芽孢杆菌分离蛋白Spo0J(PARB)参与了这两个过程，但其功能尚不清楚。我们发现，Spo0J结合到其起源-近端结合位点(PARS)，招募了染色体(SMC)复合体的结构维持到起源。我们证明，在没有Spo0J或PARS位点的情况下，SMC复合体的亚细胞定位被破坏。此外，SMC复合体与Spo0J共定位在末端附近的PARS位点的起始处和插入位置，从而导致染色体组织和分离的缺陷。最后，我们发现纯化的SMC与包被Spo0J的DNA结合的亲和力高于裸露DNA。这些数据与Spo0J-PARS将SMC招募到起源地的模型一致，该模型认为SMC招募到起源区组织起源区并促进有效的染色体分离。
Organization and segregation of replicated chromosomes are essential components of cell division in all organisms. The Bacillus subtilis partitioning protein Spo0J (ParB) has been implicated in both processes but how it functions remains unclear. We have discovered that Spo0J bound to its origin-proximal binding sites (parS) recruits the Structural Maintenance of Chromosomes (SMC) complex to the origin. We demonstrate that the subcellular localization of the SMC complex is disrupted in the absence of Spo0J or the parS sites. Furthermore, the SMC complex co-localizes with Spo0J at the origin and insertion of parS sites near the terminus targets SMC to this position leading to defects in chromosome organization and segregation. Finally, we show that purified SMC binds Spo0J-coated DNA with higher affinity than naked DNA. These data are consistent with a model in which recruitment of SMC to the origin by Spo0J-parS organizes the origin region and promotes efficient chromosome segregation.
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影响因子：
3.2
作者：
Livny, Jonathan;Yamaichi, Yoshiharu;Waldor, Matthew K.
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.94.9.4721
发表时间：
1997-04-29
影响因子：
11.1
作者：
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通讯作者：
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