Root-associated bacterial communities and root metabolite composition are linked to nitrogen use efficiency in sorghum.
Root-associated bacterial communities and root metabolite composition are linked to nitrogen use efficiency in sorghum.
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DOI：
10.1128/msystems.01190-23
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发表时间：
2024-01-23
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
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发展具有高氮利用效率（NUE）的谷类作物是全球农业的首要任务。除了传统的植物育种和基因工程之外，植物微生物组的使用提供了另一种改善作物 NUE 的方法。为了深入了解与 NUE 不同的高粱品系相关的细菌群落，设计了一项田间实验，比较了充足和缺乏氮 (N) 条件下的 24 个不同的高粱品系。使用扩增子测序和非靶向气相色谱-质谱分析来表征与低氮敏感性不同的高粱基因型相关的细菌群落和根部代谢组。我们证明，氮胁迫和高粱类型（能量高粱、甜高粱和粒用高粱）显着影响高粱的根部相关细菌群落和根部代谢物组成。我们发现高粱 NUE 与根际细菌丰富度和多样性呈正相关。高 NUE 品系中更大的 α 多样性与优势细菌分类群假单胞菌丰度的减少有关。在低氮胁迫下，根代谢物和根际细菌群落之间存在多种强相关性。这表明低氮引起的高粱微生物组的变化与宿主植物的根部代谢物有关。综上所述，我们的研究结果表明，根代谢物的宿主遗传调控在定义不同 NUE 和低氮胁迫耐受性的高粱基因型的根相关微生物组中发挥着重要作用。开发氮肥利用率更高的作物对于未来增强全球农业的可持续性至关重要。这一目标主要通过植物育种和植物分子工程来实现，但这些方法只取得了有限的成功。因此，需要充分探索利用土壤微生物的不同策略，因为众所周知，土壤微生物通过多种机制改善植物生长。为了设计利用土壤微生物组来提高 NUE 的方法，首先必须了解土壤微生物、根系代谢物和作物生产力之间的关系。使用这种方法，我们在实地研究中证明了某些关键代谢物和特定微生物与高粱 NUE 的高低相关。这一重要信息为开发通过土壤微生物的积极贡献提高 NUE 的作物基因型提供了一条新的道路。
The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We demonstrated that N stress and sorghum type (energy, sweet, and grain sorghum) significantly impacted the root-associated bacterial communities and root metabolite composition of sorghum. We found a positive correlation between sorghum NUE and bacterial richness and diversity in the rhizosphere. The greater alpha diversity in high NUE lines was associated with the decreased abundance of a dominant bacterial taxon, Pseudomonas. Multiple strong correlations were detected between root metabolites and rhizosphere bacterial communities in response to low N stress. This indicates that the shift in the sorghum microbiome due to low N is associated with the root metabolites of the host plant. Taken together, our findings suggest that host genetic regulation of root metabolites plays a role in defining the root-associated microbiome of sorghum genotypes differing in NUE and tolerance to low N stress. The development of crops that are more nitrogen use-efficient (NUE) is critical for the future of the enhanced sustainability of agriculture worldwide. This objective has been pursued mainly through plant breeding and plant molecular engineering, but these approaches have had only limited success. Therefore, a different strategy that leverages soil microbes needs to be fully explored because it is known that soil microbes improve plant growth through multiple mechanisms. To design approaches that use the soil microbiome to increase NUE, it will first be essential to understand the relationship among soil microbes, root metabolites, and crop productivity. Using this approach, we demonstrated that certain key metabolites and specific microbes are associated with high and low sorghum NUE in a field study. This important information provides a new path forward for developing crop genotypes that have increased NUE through the positive contribution of soil microbes.
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影响因子：
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作者：
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