Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes.
Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes.
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DOI：
10.3389/fpls.2021.643192
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发表时间：
2021
影响因子：
5.6
通讯作者：
Gupta R
中科院分区：
文献类型：
--
作者：
Bollam S;Romana KK;Rayaprolu L;Vemula A;Das RR;Rathore A;Gandham P;Chander G;Deshpande SP;Gupta R
探索高粱的自然遗传变异并利用其提高氮素利用效率是现代作物改良计划的主要目标之一。综合策略包括高通量表型分析、基于下一代测序（NGS）的基因分型技术以及先验选择的候选基因研究，这些研究有助于了解支持这种复杂性状的详细生理和分子机制。一组60个不同的高粱基因型进行了评估，不同的营养，生殖和产量性状相关的NUE在田间（三个氮制度下）的两个赛季。不同的产量和相关性状在0和50%N下的显着变化证实了高粱在低N制度下的本地遗传变异的可用性。具有不同遗传背景的高粱基因型具有有趣的相似NUE相关性状。使用基于测序的基因分型SNP（>89 K）来研究群体结构，并且系统发育分组确定了三个不同的组。系统发育群上个体的籽粒氮和茎秆氮含量信息表明，在不同氮素水平下，个体适应性分布具有随机性。这项研究确定了有前途的高粱基因型在不同的环境下表现一致，在低氮条件下的产量缓冲能力。我们还报告了更好地执行不同的生产用途的基因型-谷物，秸秆，和两用高粱有差异的适应反应NUE性状。NUE相关基因的表达谱在地上部和根组织的对比线（PVK 801和HDW 703）生长在不同的N条件下显示有趣的结果。对照系的根组织表现出转运蛋白基因[铵转运蛋白（SbAMT）、硝酸盐转运蛋白（SbNRT）]、初级同化蛋白基因[谷氨酰胺合成酶（SbGS）、谷氨酸合酶（SbGOGAT[NADH]、SbGOGAT[Fd]）、同化蛋白基因[亚硝酸盐还原酶（SbNiR[NADH]3）]和氨基酸生物合成相关基因[谷氨酸脱氢酶（SbGDH）]的差异表达谱。在不同的N剂量下，对比高粱基因型的鉴定和表达谱分析将提供新的信息，以了解NUE基因对适应高粱差异N制度的反应。从本研究中鉴定的高氮素利用效率基因型可能是深入分子分析的潜在候选者，并有助于N高效高粱品种的开发。
Exploring the natural genetic variability and its exploitation for improved Nitrogen Use Efficiency (NUE) in sorghum is one of the primary goals in the modern crop improvement programs. The integrated strategies include high-throughput phenotyping, next generation sequencing (NGS)-based genotyping technologies, and a priori selected candidate gene studies that help understand the detailed physiological and molecular mechanisms underpinning this complex trait. A set of sixty diverse sorghum genotypes was evaluated for different vegetative, reproductive, and yield traits related to NUE in the field (under three N regimes) for two seasons. Significant variations for different yield and related traits under 0 and 50% N confirmed the availability of native genetic variability in sorghum under low N regimes. Sorghum genotypes with distinct genetic background had interestingly similar NUE associated traits. The Genotyping-By-Sequencing based SNPs (>89 K) were used to study the population structure, and phylogenetic groupings identified three distinct groups. The information of grain N and stalk N content of the individuals covered on the phylogenetic groups indicated randomness in the distribution for adaptation under variable N regimes. This study identified promising sorghum genotypes with consistent performance under varying environments, with buffer capacity for yield under low N conditions. We also report better performing genotypes for varied production use—grain, stover, and dual-purpose sorghum having differential adaptation response to NUE traits. Expression profiling of NUE associated genes in shoot and root tissues of contrasting lines (PVK801 and HDW703) grown in varying N conditions revealed interesting outcomes. Root tissues of contrasting lines exhibited differential expression profiles for transporter genes [ammonium transporter (SbAMT), nitrate transporters (SbNRT)]; primary assimilatory (glutamine synthetase (SbGS), glutamate synthase (SbGOGAT[NADH], SbGOGAT[Fd]), assimilatory genes [nitrite reductase (SbNiR[NADH]3)]; and amino acid biosynthesis associated gene [glutamate dehydrogenase (SbGDH)]. Identification and expression profiling of contrasting sorghum genotypes in varying N dosages will provide new information to understand the response of NUE genes toward adaptation to the differential N regimes in sorghum. High NUE genotypes identified from this study could be potential candidates for in-depth molecular analysis and contribute toward the development of N efficient sorghum cultivars.
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影响因子：
--
作者：
Dossou-Aminon I;Loko LY;Adjatin A;Ewédjè EE;Dansi A;Rakshit S;Cissé N;Patil JV;Agbangla C;Sanni A;Akoègninou A;Akpagana K
通讯作者：
Akpagana K
影响因子：
4.9
作者：
Hirose, N;Hayakawa, T;Yamaya, T
通讯作者：
Yamaya, T
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
6.9
作者：
Chardon, Fabien;Barthelemy, Julien;Masclaux-Daubresse, Celine
通讯作者：
Masclaux-Daubresse, Celine
影响因子：
2.6
作者：
Grabowska, Agnieszka;Kwinta, Joanna;Bielawski, Wieslaw
通讯作者：
Bielawski, Wieslaw