Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans.
Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans.
复制标题
秀丽隐杆线虫脂质代谢基因的比较基因组学及功能研究
DOI：
10.1186/1471-2164-14-164
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发表时间：
2013-03-12
期刊：
影响因子：
4.4
通讯作者：
Liang B
中科院分区：
文献类型：
--
作者：
Zhang Y;Zou X;Ding Y;Wang H;Wu X;Liang B
背景
动物模型是了解脂代谢和脂代谢疾病不可缺少的基础。在过去的十年中，线虫秀丽隐杆线虫已成为一种流行的动物模型，用于探索脂质代谢，肥胖和肥胖相关疾病的调节。然而，C.对人类的影响仍然未知。在本研究中，我们系统地分析了参与脂质代谢的基因在C。elegans基因组使用比较基因组学。
结果
我们建立了一个包含471个C. elegans全基因组，并将大部分脂类基因分配到16条不同的脂类代谢途径中，整合成一个网络。70%以上的C.线虫脂类基因与人类同源，471个C. elegans脂质基因在人类、小鼠、大鼠和果蝇中是保守的，其中71个基因与人类代谢疾病特异相关。此外，使用RNA介导的干扰（RNAi）来破坏具有可用RNAi克隆的471个脂质基因中的356个的表达。我们发现，21个基因强烈影响脂肪储存，发育，生殖和其他可见的表型，其中6个以前没有参与调节脂肪代谢和其他表型。
结论
本研究首次系统性地从基因组角度研究了C. elegans，支持C.线虫作为代谢疾病研究中日益突出的模型。
Background
Animal models are indispensable to understand the lipid metabolism and lipid metabolic diseases. Over the last decade, the nematode Caenorhabditis elegans has become a popular animal model for exploring the regulation of lipid metabolism, obesity, and obese-related diseases. However, the genomic and functional conservation of lipid metabolism from C. elegans to humans remains unknown. In the present study, we systematically analyzed genes involved in lipid metabolism in the C. elegans genome using comparative genomics.
Results
We built a database containing 471 lipid genes from the C. elegans genome, and then assigned most of lipid genes into 16 different lipid metabolic pathways that were integrated into a network. Over 70% of C. elegans lipid genes have human orthologs, with 237 of 471 C. elegans lipid genes being conserved in humans, mice, rats, and Drosophila, of which 71 genes are specifically related to human metabolic diseases. Moreover, RNA-mediated interference (RNAi) was used to disrupt the expression of 356 of 471 lipid genes with available RNAi clones. We found that 21 genes strongly affect fat storage, development, reproduction, and other visible phenotypes, 6 of which have not previously been implicated in the regulation of fat metabolism and other phenotypes.
Conclusions
This study provides the first systematic genomic insight into lipid metabolism in C. elegans, supporting the use of C. elegans as an increasingly prominent model in the study of metabolic diseases.
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影响因子：
3.7
作者：
Brooks KK;Liang B;Watts JL
通讯作者：
Watts JL
秀丽隐杆线虫中不饱和脂肪酸组成的遗传调节。
影响因子：
4.5
作者：
Brock, Trisha J.;Browse, John;Watts, Jennifer L.
通讯作者：
Watts, Jennifer L.
影响因子：
4
作者：
Ferguson, PJ;Chen, S;El-Shanti, H
通讯作者：
El-Shanti, H
影响因子：
4
作者：
Golden, Andy;Liu, Jun;Cohen-Fix, Orna
通讯作者：
Cohen-Fix, Orna
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J