Analyte Enrichment via Ion Concentration Polarization with Hydrogel Plugs Polymerized in PDMS Microchannels by a Facile and Comprehensive Method for Improved Polymerization.
Analyte Enrichment via Ion Concentration Polarization with Hydrogel Plugs Polymerized in PDMS Microchannels by a Facile and Comprehensive Method for Improved Polymerization.
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DOI：
10.1021/acs.analchem.2c01394
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发表时间：
2022-11-15
影响因子：
7.4
通讯作者：
Timperman, Aaron T.
中科院分区：
文献类型：
--
作者：
Chen, Dayi;Timperman, Aaron T.
将水凝胶并入微流体装置中以通过实现诸如酶固定化、样品富集和离子电流整流的过程来提供增强的功能。然而，在具有常用材料PDMS的微流体装置中，由于PDMS中的高氧浓度，水凝胶很难在环境气氛中原位溶解。即使使用非常高（1.8%）的光引发剂浓度，聚合的水凝胶也不会完全填充微通道。在这里，我们报告了一个简单的和广泛适用的协议，利用微通道预处理与20%的二苯甲酮在丙酮中，以提供一个水凝胶塞，完全填充微通道的横截面，通过消耗在PDMS基板附近的微通道壁的氧气。带负电荷的和中性的水凝胶都是由单体溶液聚合而成的，所述单体溶液利用以下的光引发剂/溶剂组合：水中的VA-086和DMSO中的二苯甲酮或IRG。光引发剂在不同浓度下和在具有不同氧化水平的装置中进行测试。使用相差显微镜表征水凝胶形态，并与水凝胶的浓缩富集和离子电流整流性能相关。采用一种新颖的方法将前体溶液限制在所需的位置，使得不需要光掩模来空间控制插塞位置。在六种水凝胶制剂中，在100 V下获得的最佳电流整流因子为~600，并且在5 min内实现的最佳分析物富集为~120倍。该方法提供了一种快速简单的方法，通过改进纳米多孔水凝胶的聚合来增加PDMS微流体装置的能力。
Hydrogels are incorporated into microfluidic devices to provide enhanced functionality by enabling processes such as enzyme immobilization, sample enrichment, and ionic current rectification. However, in the microfluidic devices with the commonly used material PDMS, hydrogels are very difficult to polymerize in situ in ambient atmosphere, because of the high oxygen concentration in PDMS. Even with very high (1.8%) photoinitiator concentrations, the polymerized hydrogel does not completely fill the microchannel. Here we report a facile and broadly applicable protocol that utilizes microchannel pretreatment with 20% benzophenone in acetone to provide a hydrogel plug that completely fills the microchannel cross-section by consuming the oxygen in the PDMS substrate near the microchannel wall. Both negatively charged and neutral hydrogels are polymerized from monomer solutions that utilize the photoinitiator/solvent combinations of: VA-086 in water, and benzophenone or IRG in DMSO. The photoinitiators were tested at different concentrations and in devices with different levels of oxidation. The hydrogel morphology is characterized using phase contrast microscopy and related to the hydrogel’s performance for concentration enrichment and ionic current rectification. A novel method is employed to confine the precursor solution in desired locations so that a photomask is not required for spatial control of the plug location. Among six hydrogel formulations, at 100 V the best current rectification factor obtained is ~600, and the best analyte enrichment achieved is ~120-fold in 5 min. This method provides a rapid and simple approach to increase the capabilities of PDMS microfluidic devices through improved polymerization of nanoporous hydrogels.
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影响因子：
3.4
作者：
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通讯作者：
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作者：
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通讯作者：
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