Effects of immunopotentiators on biochemical parameters, proinflammatory cytokine, and nonspecific immune responses in Shaoxing ducklings.
Effects of immunopotentiators on biochemical parameters, proinflammatory cytokine, and nonspecific immune responses in Shaoxing ducklings.
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免疫增强剂对绍兴鸭生化参数、促炎细胞因子和非特异性免疫反应的影响
DOI：
10.1016/j.psj.2020.08.069
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发表时间：
2020-11
期刊：
影响因子：
4.4
通讯作者：
Lu L
中科院分区：
文献类型：
--
作者：
Gu T;Li G;Wu X;Zeng T;Xu Q;Li L;Vladyslav S;Chen G;Lu L
抗生素是20世纪最重要的医学发现之一，在21世纪仍将是治疗动物和人类疾病的重要工具。然而，抗生素的滥用危害着全世界畜牧业的发展和人类健康，寻找可靠的抗生素替代品以减少抗生素的使用是非常重要的。本研究筛选了22种潜在免疫增强剂对鸭胚成纤维细胞（DEFs）凋亡和炎症因子水平的影响。结果表明，22种免疫增强剂治疗后，干扰素(IFN)-β和肿瘤坏死因子-α基因转录水平显著上调，而白细胞介素(IL)-2和Bcl2 mRNA水平显著降低。IL-1β mRNA表达量在二氢杨梅素、绿原酸、柚皮苷、咪喹莫特、胸腺苷、β- d -葡聚糖、黄芪多糖、黄芪皂苷I、黄芪黄酮、姜黄素、CpG-DNA-2和LPS处理下均显著升高。绿原酸、β- d -葡聚糖、黄芪多糖、黄芪黄酮、姜黄素、CpG-DNA-2、鸡IgG、LPS和poly（I:C）处理显著上调了caspase 3蛋白水平。综上所述，绿原酸、β- d -葡聚糖、黄芪黄酮、CpG-DNA-2和鸡IgG对鸭DEFs具有正向免疫调节作用。因此，我们选择这5种免疫增强剂来进一步验证它们在体内的免疫调节功能。结果表明，除β- d -葡聚糖外，各免疫增强剂处理大鼠血清AST活性均显著下调，血清IL12p40、IL-1β、IFN-α和IFN-β活性均显著高于对照组。5种免疫增强剂还能诱导鸭的模式识别受体和炎症因子基因表达。5种免疫增强剂均能提高血清caspase 3、iNOSm、COX2含量，降低Bcl2水平。说明这5种免疫增强剂能增强鸭的先天免疫反应。综上所述，本研究不仅筛选出了5种鸭先天免疫增强剂，而且初步阐明了它们的作用机制，为开发有效的鸭病病原感染预防方法提供了新的思路。
Antibiotics are one of the most important medical discoveries of the 20th century and will remain an essential tool for treating animal and human diseases in the 21st century. However, misuse of antibiotics imperils the development of animal husbandry and human health all over the world, and it is important to find reliable alternatives to antibiotics to reduce the use of antibiotics. In this study, 22 potential immunopotentiators were screened on the levels of apoptosis and inflammatory factor in duck embryo fibroblast cells (DEFs). The results indicated that interferon (IFN)-β and tumor necrosis factor-α gene transcriptions were significantly upregulated, while interleukin (IL)-2 and Bcl2 mRNA levels were significantly decreased during 22 immunopotentiators treatment. Besides, the expression level of IL-1β mRNA showed significant increase during dihydromyricetin, chlorogenic acid, naringin, imiquimod, thymopentin, β-D-Glucan, astragalus polysacharin, astragalus saponin I, astragalus flavone, curcumin, CpG-DNA-2, and LPS treatment. And the level of caspase 3 protein was significantly upregulated with treating chlorogenic acid, β-D-Glucan, astragalus polysacharin, astragalus flavone, curcumin, CpG-DNA-2, chicken IgG, LPS, and poly(I:C). These results indicated that chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA-2, and chicken IgG have the positive immune regulation effects on duck DEFs. Thus, the 5 immunopotentiators were chosen to further verify their immunomodulatory function in vivo. The results showed that the activity of serum AST was significantly downregulated during all immunopotentiators treatments excepting for β-D-Glucan, and the activities of serum IL12p40, IL-1β, IFN-α, and IFN-β were significantly increased compared with the control group. Five immunopotentiators also induced the duck's pattern recognition receptors and inflammatory factor gene expression. In addition, 5 immunopotentiators could facilitate the contents of serum caspase 3, iNOSm and COX2 and reduce the Bcl2. These results suggested that these 5 immunopotentiators could enhance duck innate immune responses. Taken together, our study not only screened out 5 kinds of duck innate immune immunopotentiators but also initially clarified their underlying mechanism of action, which provide a new insight for the development of efficient approaches to prevent the duck disease from pathogen infections.
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影响因子：
4.4
作者：
Liu, Weifeng;Han, Fang;Zhang, Xiaobo
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影响因子：
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作者：
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影响因子：
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Evensen, O
DOI：
10.1046/j.1439-0396.2003.00376.x
发表时间：
2003-02-01
影响因子：
2.7
作者：
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通讯作者：
Sauerwein, H