Single-cell transcriptomics illuminates regulatory steps driving anterior-posterior patterning of Drosophila embryonic mesoderm.
Single-cell transcriptomics illuminates regulatory steps driving anterior-posterior patterning of Drosophila embryonic mesoderm.
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DOI：
10.1016/j.celrep.2023.113289
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发表时间：
2023-10-31
期刊：
影响因子：
8.8
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中科院分区：
文献类型：
--
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单细胞技术有望揭示转录程序如何在胚胎发生过程中协调复杂的过程。在这里，我们应用单细胞技术和遗传分析相结合，以调查与果蝇胚胎形态发生在原肠胚的动态转录变化。我们的数据集涵盖了囊胚到原肠胚的过渡，提供了一个全面的单细胞基因表达图谱，通过遗传分析验证了细胞谱系。亚聚类和轨迹分析揭示了一个令人惊讶的逐步发展模式过渡合子基因表达和指定胚层，以及揭示了蜕皮激素信号在上皮细胞间质转化中的中胚层的早期作用。我们还显示多能祖细胞出现前原肠胚通过分析尾中胚层细胞的转录轨迹，包括衍生物，最终纳入内脏肌肉的中肠和后肠。本研究提供了一个丰富的原肠胚形成的资源，并阐明了空间调控的转录状态的时间转换过程中。Sun等人使用单细胞RNA-seq来研究整体基因表达如何进行以适应细胞变化并协调与果蝇原肠胚形成相关的形态发生运动。本研究通过对中胚层形成过程中的调控步骤的研究，揭示了在形态发生过程中调控EMT和集体细胞迁移的机制。
Single-cell technologies promise to uncover how transcriptional programs orchestrate complex processes during embryogenesis. Here, we apply a combination of single-cell technology and genetic analysis to investigate the dynamic transcriptional changes associated with Drosophila embryo morphogenesis at gastrulation. Our dataset encompassing the blastoderm-to-gastrula transition provides a comprehensive single-cell map of gene expression across cell lineages validated by genetic analysis. Subclustering and trajectory analyses revealed a surprising stepwise progression in patterning to transition zygotic gene expression and specify germ layers as well as uncovered an early role for ecdysone signaling in epithelial-to-mesenchymal transition in the mesoderm. We also show multipotent progenitors arise prior to gastrulation by analyzing the transcription trajectory of caudal mesoderm cells, including a derivative that ultimately incorporates into visceral muscles of the midgut and hindgut. This study provides a rich resource of gastrulation and elucidates spatially regulated temporal transitions of transcription states during the process. Sun et al. used single-cell RNA-seq to investigate how overall gene expression progresses to accommodate cellular changes and coordinate the morphogenetic movements associated with Drosophila gastrulation. Identifying regulatory steps involved in patterning the mesoderm, this study provides insights into the mechanisms regulating EMT and collective cell migration during morphogenesis.
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