The Iroquois complex is required in the dorsal mesoderm to ensure normal heart development in Drosophila.
The Iroquois complex is required in the dorsal mesoderm to ensure normal heart development in Drosophila.
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DOI：
10.1371/journal.pone.0076498
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pandur P
中科院分区：
文献类型：
--
作者：
Mirzoyan Z;Pandur P
果蝇心脏发育是研究控制心脏发生的众多因素的协调作用的一个非常宝贵的系统。心脏祖细胞出现在特定的背侧中胚层区域，这些区域受到多种信号传导途径的时间协调作用的影响。果蝇易洛魁复合体（Iro-C）由三个同源框转录因子araucan（ara）、caupolican（caup）和mirr（mirr）组成。Iro-C已被证明参与组织图案化，导致特定结构的分化，例如外侧背和背侧头结构，并建立眼睛的背腹边界。Iro-C在心脏发生中的功能尚未研究。我们的数据表明，整个Iro复合物的损失，以及任何ara/caup或mirr的损失，影响果蝇的心脏发育。此外，数据表明，加塔因子Pannier需要Iro-C的存在才能在心脏发生中起作用。此外，对Iro-C成员的详细表达模式分析揭示了可能存在一种新的Even-skipped表达心包细胞亚群和7对以前未描述的心脏相关细胞。总之，这项工作介绍了Iro-C作为心脏正常发育所需的一组新的转录因子。由于Iro-C的成员可能起作用，至少部分地，在背侧中胚层的能力因素，我们的研究结果是根本的未来的研究，旨在破译在背侧中胚层决定不同细胞命运的因素之间的调节相互作用。
Drosophila heart development is an invaluable system to study the orchestrated action of numerous factors that govern cardiogenesis. Cardiac progenitors arise within specific dorsal mesodermal regions that are under the influence of temporally coordinated actions of multiple signaling pathways. The Drosophila Iroquois complex (Iro-C) consists of the three homeobox transcription factors araucan (ara), caupolican (caup) and mirror (mirr). The Iro-C has been shown to be involved in tissue patterning leading to the differentiation of specific structures, such as the lateral notum and dorsal head structures and in establishing the dorsal-ventral border of the eye. A function for Iro-C in cardiogenesis has not been investigated yet. Our data demonstrate that loss of the whole Iro complex, as well as loss of either ara/caup or mirr only, affect heart development in Drosophila . Furthermore, the data indicate that the GATA factor Pannier requires the presence of Iro-C to function in cardiogenesis. Furthermore, a detailed expression pattern analysis of the members of the Iro-C revealed the presence of a possibly novel subpopulation of Even-skipped expressing pericardial cells and seven pairs of heart-associated cells that have not been described before. Taken together, this work introduces Iro-C as a new set of transcription factors that are required for normal development of the heart. As the members of the Iro-C may function, at least partly, as competence factors in the dorsal mesoderm, our results are fundamental for future studies aiming to decipher the regulatory interactions between factors that determine different cell fates in the dorsal mesoderm.
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