Genetic analysis of the IL8 gene polymorphism (rs4073) in generalized aggressive periodontitis.
Genetic analysis of the IL8 gene polymorphism (rs4073) in generalized aggressive periodontitis.
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DOI：
10.1016/j.archoralbio.2012.05.008
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发表时间：
2013-02
影响因子：
3
通讯作者：
de Souza, Ana Paula
中科院分区：
文献类型：
--
作者：
Andia, Denise Carleto;Letra, Ariadne;Viana Casarin, Renato Correa;Casati, Marcio Zaffalon;Peres Line, Sergio Roberto;de Souza, Ana Paula
白细胞介素（IL）-8是调节炎症反应的重要趋化因子。IL-8基因中的单核苷酸多态性（SNP）参考序列（rs）4073已显示在用脂多糖刺激后调节IL-8水平。本研究调查了IL 8 rs 4073风险等位基因A的传播模式及其与侵袭性牙周炎（AgP）的易感性在家庭和病例对照队列无关的个体从巴西人口。采用标准的聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）对13个核心家系和184名无关个体进行基因分型。采用传递不平衡检验（TDT）对家系数据集进行统计分析，采用卡方检验和多变量logistic回归模型对病例对照数据集进行统计分析。TDT分析未检测到IL 8 rs 4073等位基因在受影响和未受影响的家庭成员中过度传播的证据（等位基因T：52%;等位基因A：48%; p = 0.2252）。如何预期，分析的情况下，无关的控制显示一个显着的和负相关的年龄与AgP，但是，缺乏基因型，种族群体和广义AgP之间的关联进行了观察。SNP（rs 4073）在无关个体中与AgP无关，并且没有证据表明巴西个体中AgP家族中等位基因的过度传播。
Interleukin (IL)-8 is an important chemokine for regulation of the inflammatory response. A single nucleotide polymorphism (SNP) reference sequence (rs) 4073 in the IL8 gene has been shown to regulate IL-8 levels after stimulation with lipopolysaccharide. This study investigates the transmission pattern of the IL8 rs4073 risk allele A and its association with susceptibility to Aggressive Periodontitis (AgP) in families and in a case-control cohort of unrelated individuals from a Brazilian population. Genotyping was performed by standard polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) in 13 nuclear families and 184 unrelated subjects. Statistical analysis was performed using the transmission disequilibrium test (TDT) for the family dataset and chi-square test and multivariate logistic regression modeling for the case-control dataset. TDT analyses did not detect evidence of over transmission of IL8 rs4073 alleles in affected and unaffected family members (allele T: 52%; allele A: 48%; p = 0.2252). How expected, analyses of cases and unrelated controls showed a significant and inverse association of age with AgP; however, a lack of association between genotypes, ethnic groups and generalized AgP was observed. The SNP (rs4073) was not associated with AgP in unrelated individuals and there is no evidence of over transmission of the alleles in families with AgP, from Brazilian individuals.
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影响因子：
3.7
作者：
Hildebrandt MA;Komaki R;Liao Z;Gu J;Chang JY;Ye Y;Lu C;Stewart DJ;Minna JD;Roth JA;Lippman SM;Cox JD;Hong WK;Spitz MR;Wu X
通讯作者：
Wu X
影响因子：
5.2
作者：
Horvath, S;Xu, X;Laird, NM
通讯作者：
Laird, NM
影响因子：
3.8
作者：
Garza-Gonzalez E;Bosques-Padilla FJ;Mendoza-Ibarra SI;Flores-Gutierrez JP;Maldonado-Garza HJ;Perez-Perez GI
通讯作者：
Perez-Perez GI
DOI：
10.1136/bjo.2007.123190
发表时间：
2008-04
期刊：
The British journal of ophthalmology
影响因子：
--
作者：
Goverdhan SV;Ennis S;Hannan SR;Madhusudhana KC;Cree AJ;Luff AJ;Lotery AJ
通讯作者：
Lotery AJ
影响因子：
4.3
作者：
ALBANDAR, JM;BUISCHI, YAP;BARBOSA, MFZ
通讯作者：
BARBOSA, MFZ