Analysis of IgG4 class switch-related molecules in IgG4-related disease.
Analysis of IgG4 class switch-related molecules in IgG4-related disease.
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DOI：
10.1186/ar3924
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发表时间：
2012-07-23
影响因子：
4.9
通讯作者：
Sumida T
中科院分区：
文献类型：
--
作者：
Tsuboi H;Matsuo N;Iizuka M;Tsuzuki S;Kondo Y;Tanaka A;Moriyama M;Matsumoto I;Nakamura S;Sumida T
免疫球蛋白G4（IgG 4）相关疾病（IgG 4-RD）是一种新的疾病实体，其特征是高血清IgG 4水平，IgG 4阳性浆细胞浸润和各种器官的纤维化。本研究的目的是确定IgG 4-RD中IgG 4类别转换重组的上调机制。我们从IgG 4-RD（n = 6）、干燥综合征（SS）（n = 6）和健康对照（n = 8）患者的外周血单核细胞（PBMC）中提取RNA，从从IgG 4-RD（n = 3）、SS（n = 4）和健康对照（n = 4）患者的PBMC中分选的CD 3阳性T细胞和CD 20阳性B细胞中提取RNA，以及来自IgG 4-RD（n = 11）、SS（n = 13）患者和健康对照（n = 3）的唇唾液腺（LSG）。用定量PCR检测IgG 4特异性类转换相关分子如Th 2细胞因子（IL-4和IL-13）、Treg细胞因子（IL-10和TGF-β）和转录因子（GATA 3和Foxp 3）的mRNA表达水平。还检查了IgG 4非特异性类别转换相关分子，如CD 40、CD 154、BAFF、APRIL、IRF 4和AID。IgG 4-RD的LSG中Treg细胞因子（IL-10和TGF-β）和AID的表达水平显著高于SS和对照组（均P < 0.05）。IgG 4-RD组PBMC中CD 40和CD 154的表达均显著低于SS组（P < 0.05），而CD 20阳性B细胞中的CD 40和CD 3阳性T细胞中的CD 154在三组间无显著差异。LSGs中IL-10、TGF-β和AID的过度表达可能在IgG 4-RD的发病机制中发挥重要作用，如IgG 4特异性类别转换重组和纤维化。IgG 4类别转换重组似乎主要在受影响的器官中上调。
Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a new disease entity characterized by high serum IgG4 levels, IgG4-positive plasmacytic infiltration, and fibrosis in various organs. The purpose of this study was to determine the mechanism of upregulation of IgG4 class switch recombination in IgG4-RD. We extracted RNA from peripheral blood mononuclear cells (PBMCs) of patients with IgG4-RD (n = 6), Sjögren syndrome (SS) (n = 6), and healthy controls (n = 8), from CD3-positive T cells and CD20-positive B cells sorted from PBMCs of patients with IgG4-RD (n = 3), SS (n = 4), and healthy controls (n = 4), as well as from labial salivary glands (LSGs) of patients with IgG4-RD (n = 11), SS (n = 13), and healthy controls (n = 3). The mRNA expression levels of IgG4-specific class switch-related molecules, such as Th2 cytokines (IL-4 and IL-13), Treg cytokines (IL-10 and TGF-β), and transcriptional factors (GATA3 and Foxp3) were examined with quantitative polymerase chain reaction (PCR). IgG4-nonspecific class switch-related molecules, such as CD40, CD154, BAFF, APRIL, IRF4, and AID, were also examined. The expression levels of Treg cytokines (IL-10 and TGF-β) and AID were significantly higher in LSGs of IgG4-RD than in SS and the controls (P < 0.05, each). In contrast, those of CD40 and CD154 were significantly lower in PBMCs of IgG4-RD than in SS (P < 0.05, each), whereas CD40 in CD20-positive B cells and CD154 in CD3-positive T cells were comparable in the three groups. Overexpression of IL-10, TGF-β, and AID in LSGs might play important roles in the pathogenesis of IgG4-RD, such as IgG4-specific class-switch recombination and fibrosis. IgG4 class-switch recombination seems to be mainly upregulated in affected organs.
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DOI：
10.1158/1078-0432.ccr-08-1845
发表时间：
2009-05-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Shaffer AL;Emre NC;Romesser PB;Staudt LM
通讯作者：
Staudt LM
一种新的临床实体，IgG4 相关疾病 (IgG4RD)：一般概念和细节。
影响因子：
2.2
作者：
Umehara, Hisanori;Okazaki, Kazuichi;Masaki, Yasufumi;Kawano, Mitsuhiro;Yamamoto, Motohisa;Saeki, Takako;Matsui, Shoko;Sumida, Takayuki;Mimori, Tsuneyo;Tanaka, Yoshiya;Tsubota, Kazuo;Yoshino, Tadashi;Kawa, Shigeyuki;Suzuki, Ritsuro;Takegami, Tsutomu;Tomosugi, Naohisa;Kurose, Nozomu;Ishigaki, Yasuhito;Azumi, Atsushi;Kojima, Masaru;Nakamura, Shigeo;Inoue, Dai
通讯作者：
Inoue, Dai
DOI：
10.3109/08977194.2011.595714
发表时间：
2011-10
期刊：
Growth factors (Chur, Switzerland)
影响因子：
--
作者：
Biernacka A;Dobaczewski M;Frangogiannis NG
通讯作者：
Frangogiannis NG
影响因子：
2.9
作者：
Miyoshi, Hideaki;Uchida, Kazushige;Okazaki, Kazuichi
通讯作者：
Okazaki, Kazuichi
影响因子：
2.2
作者：
Miyake, Katsuhisa;Moriyama, Masafumi;Nakamura, Seiji
通讯作者：
Nakamura, Seiji