Mold contamination in a controlled hospital environment: a 3-year surveillance in southern Italy.
Mold contamination in a controlled hospital environment: a 3-year surveillance in southern Italy.
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DOI：
10.1186/s12879-014-0595-z
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发表时间：
2014-11-15
影响因子：
3.7
通讯作者：
Montagna MT
中科院分区：
文献类型：
--
作者：
Caggiano G;Napoli C;Coretti C;Lovero G;Scarafile G;De Giglio O;Montagna MT
空气中丝状真菌的环境监测是必要的，以减少在手术室和控制环境中的真菌浓度，并防止感染。本研究报告的丝状真菌的监测结果进行了手术室和控制环境中的空气和表面的样品在意大利大学医院。2010年1月至2012年12月期间，在32个手术室和5个有高风险患者的科室进行了抽样。使用微生物空气采样器对室内空气样本进行采样;使用罗氏接触板进行表面采样。在属和种的水平上鉴定了真菌分离物。61份样本（61/465; 13.1%）为霉菌阳性，其中18份来自受控环境（18/81; 22.2%），43份（43/384; 11.2%）来自手术室。最高的空气真菌负荷（AFL，菌落形成单位每立方米[CFU/m3]）记录在眼科手术室，而儿科肿瘤血液病房AFL最高的病房（47 CFU/m3）。从空气标本培养中鉴定出的最常见真菌是曲霉属。(91.8%）、青霉属（Penicillium spp.）（6%）和拟青霉属（Paecilomyces spp.）(1.5%).在研究期间，在空气控制环境中记录到CFU随时间的统计学显著增加（p = 0.043），而手术室中AFL的增加无统计学显著性（p = 0.145）。霉菌被发现在29.1%的样品从表面获得。烟曲霉是最常见的分离（68.5%）。我们的研究结果将成为旨在改善这些特殊病房的空气和表面质量的行动的基础。缺乏任何遗传分析阻止了真菌环境污染与真菌感染发作的任何相关性，将在同一医院收治的患者中进行前瞻性研究。本文的在线版本（doi：10.1186/s12879-014-0595-z）包含补充材料，可供授权用户使用。
Environmental monitoring of airborne filamentous fungi is necessary to reduce fungal concentrations in operating theaters and in controlled environments, and to prevent infections. The present study reports results of a surveillance of filamentous fungi carried out on samples from air and surfaces in operating theaters and controlled environments in an Italian university hospital. Sampling was performed between January 2010 and December 2012 in 32 operating theaters and five departments with high-risk patients. Indoor air specimens were sampled using a microbiological air sampler; Rodac contact plates were used for surface sampling. Fungal isolates were identified at the level of genera and species. Sixty-one samples (61/465; 13.1%) were positive for molds, with 18 from controlled environments (18/81; 22.2%) and 43 (43/384; 11.2%) from operating theaters. The highest air fungal load (AFL, colony-forming units per cubic meter [CFU/m3]) was recorded in the ophthalmology operating theater, while the pediatric onco-hematology ward had the highest AFL among the wards (47 CFU/m3). The most common fungi identified from culture of air specimens were Aspergillus spp. (91.8%), Penicillium spp., (6%) and Paecilomyces spp. (1.5%). During the study period, a statistically significant increase in CFU over time was recorded in air-controlled environments (p = 0.043), while the increase in AFL in operating theaters was not statistically significant (p = 0.145). Molds were found in 29.1% of samples obtained from surfaces. Aspergillus fumigatus was the most commonly isolated (68.5%). Our findings will form the basis for action aimed at improving the air and surface quality of these special wards. The lack of any genetic analysis prevented any correlation of fungal environmental contamination with onset of fungal infection, an analysis that will be undertaken in a prospective study in patients admitted to the same hospital. The online version of this article (doi:10.1186/s12879-014-0595-z) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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通讯作者：
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作者：
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