Assessment of Inner Blood-Retinal Barrier: Animal Models and Methods.
Assessment of Inner Blood-Retinal Barrier: Animal Models and Methods.
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作者：
神经视网膜的正常功能依赖于血视网膜屏障（BRB）调节的独特视网膜环境，该屏障限制溶质、液体和有毒物质的通过。 BRB 损伤发生在许多视网膜血管疾病中，并且 BRB 的分解对疾病病理学有显着影响。因此，了解 BRB 发育和维持所涉及的不同分子成分和信号通路对于开发治疗方式至关重要。本综述总结了参与内层BRB（iBRB）形成和维持的主要分子信号通路，以及视网膜血管渗漏眼病的代表性动物模型。重点关注 Wnt/β-连环蛋白信号传导的研究，这对于视网膜和脑血管血管生成和屏障形成至关重要。此外，还描述了多种用于检测和分析血管渗漏的体内和体外方法及其优点和局限性。这些临床前动物模型和评估 iBRB 的方法为描述视网膜血管疾病的分子机制和评估治疗药物提供了有价值的实验工具。
Proper functioning of the neural retina relies on the unique retinal environment regulated by the blood–retinal barrier (BRB), which restricts the passage of solutes, fluids, and toxic substances. BRB impairment occurs in many retinal vascular diseases and the breakdown of BRB significantly contributes to disease pathology. Understanding the different molecular constituents and signaling pathways involved in BRB development and maintenance is therefore crucial in developing treatment modalities. This review summarizes the major molecular signaling pathways involved in inner BRB (iBRB) formation and maintenance, and representative animal models of eye diseases with retinal vascular leakage. Studies on Wnt/β-catenin signaling are highlighted, which is critical for retinal and brain vascular angiogenesis and barriergenesis. Moreover, multiple in vivo and in vitro methods for the detection and analysis of vascular leakage are described, along with their advantages and limitations. These pre-clinical animal models and methods for assessing iBRB provide valuable experimental tools in delineating the molecular mechanisms of retinal vascular diseases and evaluating therapeutic drugs.
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影响因子：
7.3
作者：
Benson K;Cramer S;Galla HJ
通讯作者：
Galla HJ
颈总动脉注射荧光微球后小鼠大脑微梗塞的组织化学
影响因子：
6.1
作者：
Shen Y;Yao MJ;Su YX;Xu DS;Wang J;Wang GR;Cui JJ;Zhang JL;Bai WZ
通讯作者：
Bai WZ
影响因子：
4.6
作者：
Ebrahimiadib, Nazanin;Kianzad, Zahra;Zarei, Mohammad;Davoudi, Samaneh;Riazi-Esfahani, Hamid;Bazvand, Fatemeh;Mahdizad, Zahra;Modjtahedi, Bobeck S.
通讯作者：
Modjtahedi, Bobeck S.
影响因子：
9.8
作者：
Ai, MR;Heeger, S;Schelling, DK
通讯作者：
Schelling, DK
DOI：
10.1073/pnas.0808698106
发表时间：
2009-02-10
影响因子：
11.1
作者：
Argaw, Azeb Tadesse;Gurfein, Blake T.;John, Gareth R.
通讯作者：
John, Gareth R.