Applications of high-resolution magic angle spinning MRS in biomedical studies I-cell line and animal models.
Applications of high-resolution magic angle spinning MRS in biomedical studies I-cell line and animal models.
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DOI：
10.1002/nbm.3700
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发表时间：
2017-06
影响因子：
2.9
通讯作者：
Cheng LL
中科院分区：
文献类型：
--
作者：
Kaebisch E;Fuss TL;Vandergrift LA;Toews K;Habbel P;Cheng LL
高分辨率魔角旋转(HRMAS)磁共振波谱(MRS)允许直接测量非液体组织和细胞样本，以提供对生理和病理过程的细胞代谢的有价值的见解。HRMAS产生的高分辨率光谱与从样品提取物的溶液中获得的光谱相当，但没有复杂的代谢物提取过程，并保留了适合光谱分析后进行病理检查的组织细胞结构。该技术已被广泛应用于生物医学和生化研究，并成为代谢组学研究的主要平台之一。通过对单个代谢物、代谢物比率或代谢谱进行整体量化，HRMAS为诊断和预测各种疾病的临床结果以及破译药物治疗或异物相互作用引起的代谢变化提供了很有希望的可能性。本文对文献报道的动物模型和细胞系的HRMAS MRS结果进行了评价，并介绍了HRMAS方法在了解病理过程、治疗的有效性、疾病动物模型的发展以及HRMAS方法学的新进展方面的各种应用。高分辨率魔角旋转(HRMAS)磁共振波谱(MRS)是一种提高非液体生物样品分辨率的方法。自1996年引入以来，HRMAS已被广泛应用于生物医学和生化研究，是代谢组学分析的主要技术之一。在这篇综述中，对动物模型和细胞系的HRMAS结果进行了评估，讨论了HRMAS在理解病理过程、治疗效果、疾病动物模型开发和方法学进展方面的应用。
High-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS) allows for direct measurements of non-liquid tissue and cell specimens to present valuable insights of the cellular metabolisms of physiological and pathological processes. HRMAS produces high resolution spectra comparable to those obtained from solutions of specimen extracts but without complex metabolite extraction processes, and preserves the tissue cellular structure suitable for pathological examinations following spectroscopic analysis. The technique has been applied in a wide variety of biomedical and biochemical studies and become one of the major platforms of metabolomic studies. By quantifying single metabolites, metabolite ratios or metabolic profiles in their entirety, HRMAS presents promising possibilities for diagnosis and prediction of clinical outcomes for various diseases, as well as deciphering of metabolic changes resulting from drug therapies or xenobiotic interactions. In this review, we evaluate HRMAS MRS results on animal models and cell lines reported in literature, and present the diverse applications of the method for the understanding of pathological processes, the effectiveness of therapies, the developments of disease animal models, and the new progresses in the HRMAS methodology. High resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS) is a method which enhances resolution for non-liquid biological samples. Since its introduction in 1996, HRMAS has been used in a wide variety of biomedical and biochemical studies and is one of the major techniques used in metabolomic analysis. In this review, HRMAS results on animal models and cell lines are evaluated, with discussions of applications for understanding pathological processes, therapy effectiveness, disease animal model development, and methodology progress.
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影响因子：
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