Overexpression of four MiTFL1 genes from mango delays the flowering time in transgenic Arabidopsis.
Overexpression of four MiTFL1 genes from mango delays the flowering time in transgenic Arabidopsis.
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芒果中四个 MiTFL1 基因的过度表达延迟了转基因拟南芥的开花时间
DOI：
10.1186/s12870-021-03199-9
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发表时间：
2021-09-07
影响因子：
5.3
通讯作者：
Luo C
中科院分区：
文献类型：
--
作者：
Wang YH;He XH;Yu HX;Mo X;Fan Y;Fan ZY;Xie XJ;Liu Y;Luo C
背景TERMINAL FLOWER 1 (TFL1) 属于磷脂酰乙醇胺结合蛋白 (PEBP) 家族，参与花序分生组织的发育并抑制多种植物的开花。本研究从芒果品种‘思吉米’中克隆了4个TFL1基因，分别命名为MiTFL1-1、MiTFL1-2、MiTFL1-3和MiTFL1-4。结果序列分析表明，编码的MiTFL1蛋白含有保守的PEBP结构域，属于TFL1类群。表达分析表明MiTFL1基因不仅在营养器官中表达，而且在生殖器官中表达，且表达水平与花的发育相关。四个 MiTFL1 基因的过度表达延迟了转基因拟南芥的开花。此外，MiTFL1-1和MiTFL1-3改变了一些转基因植物的花形态。酵母双杂交（Y2H）分析表明，几种胁迫相关蛋白与MiTFL1蛋白相互作用。结论四个MiTFL1基因表现出相似的表达模式，在拟南芥中过度表达导致开花延迟。此外，MiTFL1-1和MiTFL1-3的过表达影响花器官的发育。此外，MiTFL1 蛋白可以与 bHLH 和 14-3-3 蛋白相互作用。这些结果表明MiTFL1基因可能在芒果的开花过程中发挥重要作用。
BackgroundTERMINAL FLOWER 1(TFL1) belongs to the phosphatidylethanolamine-binding protein (PEBP) family, which is involved in inflorescence meristem development and represses flowering in several plant species. In the present study, fourTFL1genes were cloned from the mango (Mangifera indicaL.) variety ‘SiJiMi’ and namedMiTFL1-1,MiTFL1-2,MiTFL1-3andMiTFL1-4.ResultsSequence analysis showed that the encoded MiTFL1 proteins contained a conserved PEBP domain and belonged to the TFL1 group. Expression analysis showed that theMiTFL1genes were expressed in not only vegetative organs but also reproductive organs and that the expression levels were related to floral development. Overexpression of the fourMiTFL1genes delayed flowering in transgenicArabidopsis. Additionally,MiTFL1-1andMiTFL1-3changed the flower morphology in some transgenic plants. Yeast two-hybrid (Y2H) analysis showed that several stress-related proteins interacted with MiTFL1 proteins.ConclusionsThe fourMiTFL1genes exhibited a similar expression pattern, and overexpression inArabidopsisresulted in delayed flowering. Additionally,MiTFL1-1andMiTFL1-3overexpression affected floral organ development. Furthermore, the MiTFL1 proteins could interact with bHLH and 14-3-3 proteins. These results indicate that theMiTFL1genes may play an important role in the flowering process in mango.
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影响因子：
7.2
作者：
Luccioni, Laura;Krzymuski, Martin;Casal, Jorge J.
通讯作者：
Casal, Jorge J.
影响因子：
5.1
作者：
Jose Carmona, Maria;Calonje, Myriam;Miguel Martinez-Zapater, Jose
通讯作者：
Miguel Martinez-Zapater, Jose
影响因子：
1.5
作者：
Luo, C.;He, X. -H.;Ou, S. -J.
通讯作者：
Ou, S. -J.
影响因子：
5.1
作者：
Gao, Yaohui;Gao, Yike;Zhang, Qixiang
通讯作者：
Zhang, Qixiang
影响因子：
6.2
作者：
Liu, Xiang;Zhang, Jian;Xiang, Qiu-Yun (Jenny)
通讯作者：
Xiang, Qiu-Yun (Jenny)