New mouse lines for the analysis of neuronal morphology using CreER(T)/loxP-directed sparse labeling.
New mouse lines for the analysis of neuronal morphology using CreER(T)/loxP-directed sparse labeling.
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DOI：
10.1371/journal.pone.0007859
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发表时间：
2009-11-16
期刊：
影响因子：
3.7
通讯作者：
Nathans J
中科院分区：
文献类型：
--
作者：
Badea TC;Hua ZL;Smallwood PM;Williams J;Rotolo T;Ye X;Nathans J
通过依赖他莫昔芬激活Cre-雌激素受体配体结合域融合蛋白[Creer(T)]对Cre介导的重组进行药理学控制被广泛用于修饰和/或可视化小鼠的细胞。在这里，我们描述了两个新的小鼠品系，通过针对rosa26基因座的基因打靶来促进Cre介导的细胞修饰。在稀疏标记实验的背景下，这些线应该被证明特别有用。R26rtTACreER系在四环素反向反式激活因子(RTTA)的转录控制下提供了普遍存在的Creer的表达；由多西环素和他莫昔芬的双重控制提供了更大的Cre介导的重组活性的动态范围。R26IAP系提供高效的Cre介导的人胎盘碱性磷酸酶(HPLAP)激活，补充了广泛使用但效率较低的Z/AP系。通过与指导细胞类型特异性CRER表达的小鼠品系杂交，R26IAP品系已被用于制作小鼠大脑中标记的胆碱能和儿茶酚胺能神经元的图谱。R26IAP系还被用来显示视网膜多巴胺能无长突细胞的完整形态，这些细胞是哺乳动物视网膜中最大的神经元之一。这里描述的两个新的小鼠品系扩大了可用于体内受控重组和使用Cre-lox系统进行细胞标记的基因工程小鼠的谱系。
Pharmacologic control of Cre-mediated recombination using tamoxifen-dependent activation of a Cre-estrogen receptor ligand binding domain fusion protein [CreER(T)] is widely used to modify and/or visualize cells in the mouse. We describe here two new mouse lines, constructed by gene targeting to the Rosa26 locus to facilitate Cre-mediated cell modification. These lines should prove particularly useful in the context of sparse labeling experiments. The R26rtTACreER line provides ubiquitous expression of CreER under transcriptional control by the tetracycline reverse transactivator (rtTA); dual control by doxycycline and tamoxifen provides an extended dynamic range of Cre-mediated recombination activity. The R26IAP line provides high efficiency Cre-mediated activation of human placental alkaline phosphatase (hPLAP), complementing the widely used, but low efficiency, Z/AP line. By crossing with mouse lines that direct cell-type specific CreER expression, the R26IAP line has been used to produce atlases of labeled cholinergic and catecholaminergic neurons in the mouse brain. The R26IAP line has also been used to visualize the full morphologies of retinal dopaminergic amacrine cells, among the largest neurons in the mammalian retina. The two new mouse lines described here expand the repertoire of genetically engineered mice available for controlled in vivo recombination and cell labeling using the Cre-lox system.
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DOI：
10.1111/j.1460-9568.2009.06631.x
发表时间：
2009-02
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Cameron MA;Pozdeyev N;Vugler AA;Cooper H;Iuvone PM;Lucas RJ
通讯作者：
Lucas RJ
影响因子：
2.5
作者：
Bin Lin;Masland, Richard H.
通讯作者：
Masland, Richard H.
影响因子：
3
作者：
Backman, Cristina M.;Zhang, YaJun;Tomac, Andreas C.
通讯作者：
Tomac, Andreas C.
影响因子：
2.5
作者：
DACEY, DM
通讯作者：
DACEY, DM
影响因子：
1.5
作者：
Lin, Congxing;Yin, Yan;Ma, Liang
通讯作者：
Ma, Liang