Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography.

Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography.
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DOI:
10.3390/s21113652
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发表时间:
2021-05-24
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Sung Y
Sung Y
中科院分区:
其他
文献类型:
--
作者:
Juntunen C;Woller IM;Sung Y

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Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.
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