Tetraglyme coatings reduce fibrinogen and von Willebrand factor adsorption and platelet adhesion under both static and flow conditions.
Tetraglyme coatings reduce fibrinogen and von Willebrand factor adsorption and platelet adhesion under both static and flow conditions.
复制标题
DOI：
10.1002/jbm.a.32085
复制
发表时间：
2009-06
影响因子：
4.9
通讯作者：
Horbett, Thomas A.
中科院分区：
文献类型：
--
作者：
Zhang, Min;Horbett, Thomas A.
先前的研究已经表明，射频等离子体沉积的四甘醇二甲醚涂层大大降低了来自高度稀释的血浆（0.1%和1%）的纤维蛋白原吸附（ΓFg）以及随后在静态条件下的血小板粘附。在这项研究中，四甘醇二甲醚的蛋白质抵抗特性与高浓度血浆重新检查，随后血小板粘附在静态和流动条件下进行了测量。本文还研究了流动条件下四甘醇二甲醚对vWf吸附的阻力（ΓvWf）及其在血小板粘附中的作用。用125 I放射性标记的蛋白质测量ΓFg和ΓvWf。通过使血小板/红细胞悬浮液通过GlycoTech流动室，在50或500 s-1的剪切速率下进行流动研究。当从一系列增加的血浆浓度中吸附时，两种蛋白质对四甘醇二甲醚的吸附稳定增加，并且在中间稀释度下未显示峰，即不存在Vroman效应。当血浆浓度小于10%时，四甘醇二甲醚表面是高度不结垢的，在静态和流动条件下均表现出超低的IFg（小于5 ng/cm 2）和极低的血小板粘附。然而，当从100%血浆进行吸附时，IFg高得多（~ 85 ng/cm 2），表明四甘醇二甲醚表面在生理环境中可能不具有足够的蛋白质抗性。为了将流动下的血小板粘附与ΓFg和ΓvWf相关联，通过改变沉积功率来产生一系列醚含量和蛋白质吸附不同的四甘醇二甲醚表面。在低剪切速率下，血小板粘附仅取决于ΓFg的量，而在高剪切速率下，ΓFg和ΓvWf均影响血小板粘附。特别地，发现必须将TvWf降低至小于0.4ng/cm 2以在高剪切下实现超低血小板粘附。
Previous studies have showed that radio-frequency plasma deposited tetraglyme coatings greatly reduced fibrinogen adsorption (ΓFg) from highly diluted plasmas (0.1% and 1%) and subsequent platelet adhesion under static conditions. In this study, the protein resistant properties of tetraglyme were re-examined with high-concentration plasma, and subsequent platelet adhesion was measured under both static and flow conditions. The resistance of tetraglyme to vWf adsorption (ΓvWf) and the role of vWf in platelet adhesion under flow were also investigated. ΓFg and ΓvWf were measured with 125I radiolabeled proteins. Flow studies were done at shear rates of 50 or 500 s−1 by passing a platelet/red cell suspension through a GlycoTech flow chamber. When adsorbed from a series of increasing plasma concentrations, the adsorption of both proteins to tetraglyme increased steadily, and did not show a peak at intermediate dilutions, i.e. there was no Vroman effect. When plasma concentration was less than 10%, the tetraglyme surface was highly non-fouling, exhibiting ultralow ΓFg (less than 5 ng/ cm2) and extremely low platelet adhesion under both static and flow conditions. However, when the adsorption was done from 100% plasma, ΓFg was much higher (~ 85 ng/cm2), indicating that tetraglyme surface may not be sufficiently protein-resistant in the physiological environment. To correlate platelet adhesion under flow with ΓFg and ΓvWf, a series of tetraglyme surfaces varying in ether content and protein adsorption was created by varying deposition power. On these surfaces, platelet adhesion at low shear rate depended only on the amount of ΓFg, but under high shear, both ΓFg and ΓvWf affected platelet adhesion. In particular, it was found that ΓvWf must be reduced to less than 0.4 ng/cm2 to achieve ultra low platelet adhesion under high shear.
登录
查看更多内容
DOI：
10.1002/jbm.820150311
发表时间：
1981-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
HORBETT, TA;WEATHERSBY, PK
通讯作者：
WEATHERSBY, PK
影响因子：
3.6
作者：
Tsai, WB;Horbett, TA
通讯作者：
Horbett, TA
影响因子：
3.9
作者：
Shen, MC;Wagner, MS;Horbett, TA
通讯作者：
Horbett, TA
影响因子：
20.3
作者：
Shen, Y;Romo, GM;Andrews, RK
通讯作者：
Andrews, RK
DOI：
10.1002/jbm.820250707
发表时间：
1991-07-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
IP, WF;SEFTON, MV
通讯作者：
SEFTON, MV