The Amino Acid Metabolic and Carbohydrate Metabolic Pathway Play Important Roles during Salt-Stress Response in Tomato.
The Amino Acid Metabolic and Carbohydrate Metabolic Pathway Play Important Roles during Salt-Stress Response in Tomato.
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氨基酸代谢和碳水化合物代谢途径在番茄盐胁迫反应中发挥重要作用
DOI：
10.3389/fpls.2017.01231
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发表时间：
2017
影响因子：
5.6
通讯作者：
Kou X
中科院分区：
文献类型：
--
作者：
Zhang Z;Mao C;Shi Z;Kou X
盐胁迫影响植物品质，影响植物的生产力和蓄水质量。在最近的一项研究中，我们进行了TOPSIS分析和RNA-Seq，生物信息学研究方法，并使用qRT-PCR检测关键基因。结果表明，番茄耐盐性鉴定的最佳盐处理条件为200 mM和19 d。基于RNA-Seq，我们发现番茄在盐胁迫响应过程中有17条氨基酸代谢途径和17条碳水化合物代谢途径富集在生物代谢中。在盐胁迫适应过程中，有7条氨基酸代谢途径和6条碳水化合物代谢途径显著富集。在7条氨基酸代谢途径和6条碳水化合物代谢途径中分别筛选出17条和19条关键基因。我们选择了一些关键基因进行qRT-PCR验证。结果表明，这些基因的表达与RNA-seq相同。我们发现这些重要途径和重要基因在整个盐胁迫适应过程中起着重要作用。这些结果提供了有价值的信息，提高了抗压能力，提高了植物的质量。
Salt stress affects the plant quality, which affects the productivity of plants and the quality of water storage. In a recent study, we conducted the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) analysis and RNA-Seq, bioinformatics study methods, and detection of the key genes with qRT-PCR. Our findings suggested that the optimum salt treatment conditions are 200 mM and 19d for the identification of salt tolerance in tomato. Based on the RNA-Seq, we found 17 amino acid metabolic and 17 carbohydrate metabolic pathways enriched in the biological metabolism during the response to salt stress in tomato. We found 7 amino acid metabolic and 6 carbohydrate metabolic pathways that were significantly enriched in the adaption to salt stress. Moreover, we screened 17 and 19 key genes in 7 amino acid metabolic and 6 carbohydrate metabolic pathways respectively. We chose some of the key genes for verifying by qRT-PCR. The results showed that the expression of these genes was the same as that of RNA-seq. We found that these significant pathways and vital genes occupy an important roles in a whole process of adaptation to salt stress. These results provide valuable information, improve the ability to resist pressure, and improve the quality of the plant.
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影响因子：
4.8
作者：
Cho, SG;Lee, YH;Choi, EJ
通讯作者：
Choi, EJ
影响因子：
6.9
作者：
Cuartero, J;Bolarín, MC;Moreno, V
通讯作者：
Moreno, V
影响因子：
4.3
作者：
Doskocilova, Anna;Plihal, Ondrej;Binarova, Pavla
通讯作者：
Binarova, Pavla
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
14.8
作者：
Lieberman, Raquel L.;Wustman, Brandon A.;Petsko, Gregory A.
通讯作者：
Petsko, Gregory A.