Chronic rhinosinusitis with nasal polyps is characterized by dysbacteriosis of the nasal microbiota.
Chronic rhinosinusitis with nasal polyps is characterized by dysbacteriosis of the nasal microbiota.
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DOI：
10.1038/s41598-018-26327-2
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发表时间：
2018-05-21
影响因子：
4.6
通讯作者：
Bachert C
中科院分区：
文献类型：
--
作者：
Chalermwatanachai T;Vilchez-Vargas R;Holtappels G;Lacoere T;Jáuregui R;Kerckhof FM;Pieper DH;Van de Wiele T;Vaneechoutte M;Van Zele T;Bachert C
慢性鼻鼻窦炎伴鼻息肉（CRSwNP）患者通常以哮喘合并症和鼻黏膜2型炎症为特征。粘膜微生物群被认为与炎症的持续存在有关，但相关关系尚未得到很好的界定。为了比较健康受试者与CRSwNP患者的细菌群落，我们收集了17名健康受试者、21名无哮喘的CRSwNP患者（CRSwNP−A）和20名合并哮喘的CRSwNP患者（CRSwNP+A）的鼻拭子。我们使用细菌16S rRNA的高通量测序分析了微生物群。细菌群落在三组之间存在差异。CRSwNP患者中流感嗜血杆菌显著富集，健康组中痤疮丙酸杆菌显著富集；尽管在57%的患者中存在金黄色葡萄球菌，但CRSwNP−A组中金黄色葡萄球菌含量丰富。在CRSwNP+A患者中发现大量大肠杆菌。与CRSwNP−A患者相比，CRSwNP+A患者的鼻组织表达的IgE、SE-IgE和IL-5浓度显著升高。共同培养表明，痤疮假单胞菌的生长受到流感嗜血杆菌、大肠杆菌和金黄色葡萄球菌的抑制。健康受试者的鼻腔微生物群与CRSwNP−A和CRSwNP+A患者不同。然而，健康状态下最丰富的物种不能抑制CRSwNP疾病。
Chronic rhinosinusitis with nasal polyp (CRSwNP) patients are often characterized by asthma comorbidity and a type-2 inflammation of the sinonasal mucosa. The mucosal microbiota has been suggested to be implicated in the persistence of inflammation, but associations have not been well defined. To compare the bacterial communities of healthy subjects with CRSwNP patients, we collected nasal swabs from 17 healthy subjects, 21 CRSwNP patients without asthma (CRSwNP−A), and 20 CRSwNP patients with co-morbid asthma (CRSwNP+A). We analysed the microbiota using high-throughput sequencing of the bacterial 16S rRNA. Bacterial communities were different between the three groups. Haemophilus influenzae was significantly enriched in CRSwNP patients, Propionibacterium acnes in the healthy group; Staphylococcus aureus was abundant in the CRSwNP−A group, even though present in 57% of patients. Escherichia coli was found in high amounts in CRSwNP+A patients. Nasal tissues of CRSwNP+A patients expressed significantly higher concentrations of IgE, SE-IgE, and IL-5 compared to those of CRSwNP−A patients. Co-cultivation demonstrated that P. acnes growth was inhibited by H. influenzae, E. coli and S. aureus. The nasal microbiota of healthy subjects are different from those of CRSwNP−A and CRSwNP+A patients. However, the most abundant species in healthy status could not inhibit those in CRSwNP disease.
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影响因子：
15.5
作者：
Cope EK;Goldberg AN;Pletcher SD;Lynch SV
通讯作者：
Lynch SV
影响因子：
2
作者：
HAMILTON, WD
通讯作者：
HAMILTON, WD
中国慢性鼻窦炎伴鼻息肉患者细菌定植与炎症模式的关系
影响因子：
12.4
作者：
Ba, L.;Zhang, N.;Bachert, C.
通讯作者：
Bachert, C.
影响因子：
3.7
作者：
Boase S;Foreman A;Cleland E;Tan L;Melton-Kreft R;Pant H;Hu FZ;Ehrlich GD;Wormald PJ
通讯作者：
Wormald PJ
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Wormald, Peter John
通讯作者：
Wormald, Peter John