The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection.
The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection.
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DOI：
10.1186/1471-2334-13-210
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发表时间：
2013-05-08
影响因子：
3.7
通讯作者：
Wormald PJ
中科院分区：
文献类型：
--
作者：
Boase S;Foreman A;Cleland E;Tan L;Melton-Kreft R;Pant H;Hu FZ;Ehrlich GD;Wormald PJ
细菌和真菌被认为影响慢性鼻窦炎（CRS）的粘膜炎症。然而，它们的存在和与疾病的关系是有争议的。本研究采用多种检测方法比较健康和患病鼻窦粘膜中的微生物多样性和微生物丰度。当代检测方法的实用性也进行了研究。分析了38例CRS和6例对照的鼻窦粘膜。细菌和真菌的分析进行了常规培养，分子诊断（聚合酶链反应与电喷雾电离飞行时间质谱）和荧光原位杂交。在所有样品（包括对照样品）中均检测到微生物，并且通常为多微生物。CRS组检出33种不同的细菌，对照组检出5种，厌氧菌检出率较高。金黄色葡萄球菌和痤疮丙酸杆菌分别是CRS和对照组中最常见的微生物。使用模式生物，FISH的敏感性为78%，特异性为93%。在CRS和对照中均检测到许多种属，然而，微生物丰度与疾病表现相关。这项研究强调了健康和患病鼻窦中微生物变异的一些基础。虽然健康的鼻窦显然不是无菌的，但生物体的流行和丰富似乎是决定疾病的关键。来自高灵敏度技术的证据将真菌在CRS中的作用限制在一小群患者中。与分子分析的比较表明，FISH和培养的检测阈值与生物丰度有关，此外，培养倾向于选择快速生长的生物。
Bacteria and fungi are believed to influence mucosal inflammation in chronic rhinosinusitis (CRS). However their presence and relationship to disease is debated. This study used multiple detection methods to compare microbial diversity and microbial abundance in healthy and diseased sinonasal mucosa. The utility of contemporary detection methods is also examined. Sinonasal mucosa was analyzed from 38 CRS and 6 controls. Bacterial and fungal analysis was performed using conventional culture, molecular diagnostics (polymerase chain reaction coupled with electrospray ionization time-of-flight mass spectrometry) and fluorescence in situ hybridization. Microbes were detected in all samples, including controls, and were often polymicrobial. 33 different bacterial species were detected in CRS, 5 in control patients, with frequent recovery of anaerobes. Staphylococcus aureus and Propionibacterium acnes were the most common organisms in CRS and controls, respectively. Using a model organism, FISH had a sensitivity of 78%, and a specificity of 93%. Many species were detected in both CRS and controls however, microbial abundance was associated with disease manifestation. This study highlights some cornerstones of microbial variations in healthy and diseased paranasal sinuses. Whilst the healthy sinus is clearly not sterile, it appears prevalence and abundance of organisms is critical in determining disease. Evidence from high-sensitivity techniques, limits the role of fungi in CRS to a small group of patients. Comparison with molecular analysis suggests that the detection threshold of FISH and culture is related to organism abundance and, furthermore, culture tends to select for rapidly growing organisms.
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影响因子：
2.6
作者：
Foreman, Andrew;Singhal, Deepti;Wormald, Peter-John
通讯作者：
Wormald, Peter-John
DOI：
10.1097/moo.0b013e32831de91e
发表时间：
2009-02-01
影响因子：
1.6
作者：
Ebbens, Fenna A.;Georgalas, Christos;Fokkens, Wytske J.
通讯作者：
Fokkens, Wytske J.
影响因子：
3.4
作者：
Healy, David Y.;Leid, Jeff G.;Hunsaker, Darrell H.
通讯作者：
Hunsaker, Darrell H.
影响因子：
9.4
作者：
Davies, CE;Hill, KE;Thomas, DW
通讯作者：
Thomas, DW
影响因子：
12.4
作者：
Foreman, A.;Holtappels, G.;Bachert, C.
通讯作者：
Bachert, C.