Effect of nutritional repletion on pituitary and serum follicle-stimulating hormone isoform distribution in growth-retarded lambs.
Effect of nutritional repletion on pituitary and serum follicle-stimulating hormone isoform distribution in growth-retarded lambs.
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营养补充对生长迟缓羔羊垂体和血清促卵泡激素亚型分布的影响。
DOI：
10.1095/biolreprod46.5.964
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发表时间：
1992
影响因子：
3.6
通讯作者：
Beitins,IZ
中科院分区：
文献类型：
--
作者：
Padmanabhan,V;Reno,KM;Borondy,M;Landefeld,TD;Ebling,FJ;Foster,DL;Beitins,IZ
我们用营养受限的去势羔羊验证了这样的假设：营养调节的GnRH分泌增加(用黄体生成素的搏动性来评估)不仅改变了垂体和血清中存在的FSH的数量，而且还改变了垂体和Scrum的FSH亚型的分布。对11只12周龄断奶羔羊进行营养限制并切除卵巢。从56wk开始，6只自由喂养14天，其余5只继续限制饮食。在摘除垂体前，频繁采集颈静脉血(间隔12分钟)4h。测定血清和脑垂体提取液中免疫反应阳性的绵羊黄体生成素(i-olh)和免疫反应阳性的绵羊卵泡刺激素(i-oFSH)浓度。测定血清和脑垂体提取液中生物活性(B)的卵泡刺激素(FSH)和I-oFSH亚型的分布模式。自由采食增加了I-OLH的搏动性和垂体及血清中I-oFSH和B-oFSH的平均浓度。营养受限动物和复种羔羊脑下垂体中i-oFSH亚型的分布模式没有不同，两者的主要FSH峰都在pH 3.5-5.6范围内洗脱。在随意喂养的动物的血清中，I-oFSH亚型也明显存在类似的优势。这种优势在3只限制喂养的动物中没有表现出来，这是因为循环中FSH的低浓度(<；2.5 ng/ml)。随后的研究，利用另外4只限饲羔羊的血清，循环i-oFSH浓度在4-14 ng/ml范围内(但没有检测到促黄体生成素脉冲)，发现在pH 3.5-5.6范围内，oFSH亚型具有类似的优势。在不同pH范围内洗脱的FSH亚型具有不同的免疫活性和生物学活性。综上所述，我们的结果表明，营养调节的内源性GnRH分泌的变化不会改变FSH亚型在限制喂养和随意喂养的去卵巢羔羊脑垂体或循环中的分布模式。
Using nutritionally restricted ovariectomized lambs, we tested the hypothesis that nutritionally regulated endogenous in-creases in GnRH secretion (as assessed by LH pulsatility) not only alter the quantity of FSH present in the pituitary and serum, but also alter the pituitary and scrum FSH isoform distribution. Eleven lambs were nutritionally restricted from weaning and ovariectomized at 12 wk of age. Beginning at 56 wk, 6 were fed ad libitum for 14 days, and the other 5 were continued on the restricted diet. Jugular blood samples were collected frequently (12-min interval) for 4 h prior to pituitary removal. Immunoreactive ovine LH (I-oLH) and immunoreactive ovine FSH (I-oFSH) concentrations were measured in sera and pituitary extracts. Bioactive (B) oFSH and I-oFSH Isoform distribution patterns were determined in serum pools and pituitary extracts. Ad libitum feeding increased I-oLH pulsatility and mean concentrations of pituitary and serum I-oFSH and B-oFSH. The I-oFSH isoform distribution patterns in the pituitaries from the nutritionally restricted animals were not different from those of repleted lambs; in both, the predominant FSH peak eluted in the pH range of 3.5–5.6. A similar predominance of I-oFSH isoforms was also evident in the serum of ad libitum-fed animals. This predominance was not demonstrable in 3 of the restricted-fed animals due to low circulating concentrations of FSH ( < 2.5 ng/ml). Subsequent studies, utilizing serum from 4 additional restricted-fed lambs with circulating I-oFSH concentrations in the range of 4–14 ng/ml (but no detectable LH pulses) revealed similar predominance of oFSH isoforms in the pH 3.5–5.6 range. The FSH isoforms eluting in different pH ranges differed in their immunological and biological activities. In summary, our results demonstrate that nutritionally regulated changes in endogenous GnRH secretion do not alter the distribution patterns of FSH isoforms in the pituitary or circulation of restricted- and ad libitum-fed ovariectomized lambs.
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小鼠左右卵巢和子宫角功能不平等。
DOI：
10.1530/jrf.0.0790125
发表时间：
1987
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
J. Wiebold;W. C. Becker
通讯作者：
W. C. Becker
小鼠产仔数和体重的单性状和拮抗指数选择。
影响因子：
3.3
作者：
E. J. Eisen
通讯作者：
E. J. Eisen
影响因子：
3.3
作者：
TESS, MW;BENNETT, GL;DICKERSON, GE
通讯作者：
DICKERSON, GE
影响因子：
3.6
作者：
POPE, WF;LAWYER, MS;FIRST, NL
通讯作者：
FIRST, NL
在选择极限下提高小鼠品系的产仔数。
DOI：
10.1017/s0016672300012246
发表时间：
1971
期刊：
Genetical research
影响因子：
--
作者：
D. Falconer
通讯作者：
D. Falconer