Norethindrone alters growth, sex differentiation and gene expression in marine medaka (Oryzias melastigma)
Norethindrone alters growth, sex differentiation and gene expression in marine medaka (Oryzias melastigma)
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炔诺酮改变海洋青鳉 (Oryzias melastigma) 的生长、性别分化和基因表达
DOI：
10.1002/tox.23477
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发表时间：
2022-01
影响因子：
4.5
通讯作者：
Zhongduo Wang
中科院分区：
文献类型：
--
作者：
Zhongdian Dong;Yuebi Chen;Xueyou Li;Ning Zhang;Yusong Guo;Yan-Qiu Liang;Zhongduo Wang
去甲thindrone （NET）是一种应用广泛的合成黄体酮，存在于水环境中，对水生生物构成威胁。本研究将海洋米鳉鱼（Oryzias melastigma）幼虫分别暴露于7.6和80.1 ng/L NET环境190 d。研究了NET对生长、性别分化、性腺组织学和下丘脑-垂体-性腺（HPG）轴相关基因转录表达谱的影响。结果表明，暴露于80.1 ng/L NET环境下，海水medaka种群为全雄，且显著降低雄性medaka的生长。暴露在7.6 ng/L NET环境中会增加海洋medaka种群的雌雄比例，降低雄性的生长，延迟雌性的卵巢成熟。然而，添加7.6或80.1 ng/L NET可加速精子成熟。在雌性中，暴露于7.6 ng/L NET后，卵巢细胞色素P450芳香化酶（cyp19a1a）和孕酮受体（pgr）、肝脏糖皮质激素受体（gr）和卵黄原蛋白（vtg）的转录水平受到抑制，可能导致卵巢成熟延迟。在雄性中，NET显著降低了雄性睾丸中促卵泡激素β (fshβ)和黄体生成素β (lhβ)、雌激素受体β (erβ)、肝脏中gr和pgr以及卵黄原蛋白受体（vtgr）的转录水平，而80.1 ng/L的NET显著上调了雄性睾丸中类固醇急性调节蛋白（star）的转录水平。这些结果表明，NET可以影响生长、性别分化和性腺成熟，并显著改变HPG轴相关基因的转录表达水平。
Norethindrone (NET) is a widely used synthetic progestin, which appears in water environments and threatens aquatic organisms. In this study, marine medaka (Oryzias melastigma) larvae were exposed to 7.6 and 80.1 ng/L NET for 190 days. The effects of NET on growth, sex differentiation, gonad histology and transcriptional expression profiles of hypothalamic‐pituitary‐gonadal (HPG) axis‐related genes were determined. The results showed that exposure to 80.1 ng/L NET caused an all‐male marine medaka population and significantly decreased the growth of males. Exposure to 7.6 ng/L NET increased the ratio of males/females in the marine medaka population, decreased the growth of males and delayed the ovary maturation in females. However, the sperm maturation was accelerated by 7.6 or 80.1 ng/L NET. In females, the transcription levels of cytochrome P450 aromatase (cyp19a1a) and progesterone receptor (pgr) in ovaries, glucocorticoid receptor (gr) and vitellogenin (vtg) in livers were suppressed after exposure to 7.6 ng/L NET, which may cause delayed ovary maturation. In males, NET significantly decreased the transcription levels of follicle stimulating hormone β (fshβ) and Luteinizing hormone β (lhβ)in the brain, Estrogen receptor β (erβ)，gr and pgr in the liver, and vitellogenin receptor (vtgr) in the testes, while NET of 80.1 ng/L led to a significant up‐regulation of steroidogenic acute regulatory protein (star) in the testes of males. These results showed that NET could influence growth, sex differentiation and gonadal maturation and significantly alter the transcriptional expression levels of HPG axis‐related genes.
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影响因子：
4.5
作者：
M. Overturf;D. Huggett
通讯作者：
M. Overturf;D. Huggett
影响因子：
4.5
作者：
T. Tran;G. MacFarlane;R. Kong;W. O'Connor;R. M. K. Yu
通讯作者：
T. Tran;G. MacFarlane;R. Kong;W. O'Connor;R. M. K. Yu
影响因子：
9.8
作者：
Chen, Shanduo;Lin, Canyuan;Leung, Jonathan Y. S.
通讯作者：
Leung, Jonathan Y. S.
DOI：
10.1016/j.cbpa.2012.02.006
发表时间：
2012-05
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
Guo-Yong Huang;G. Ying;Shan Liu;Yi-Xiang Fang
通讯作者：
Guo-Yong Huang;G. Ying;Shan Liu;Yi-Xiang Fang
影响因子：
2.7
作者：
Bin Tang;Wei Hu;Jun Hao;Zuoyan Zhu
通讯作者：
Bin Tang;Wei Hu;Jun Hao;Zuoyan Zhu