A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes.

A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes.
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DOI:
10.1007/s11103-006-9118-7
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发表时间:
2007-03
影响因子:
5.1
通讯作者:
Hirochika, Hirohiko
Hirochika, Hirohiko
中科院分区:
生物学2区
文献类型:
--
作者:
Miyao, Akio;Iwasaki, Yukimoto;Kitano, Hidemi;Itoh, Jun-Ichi;Maekawa, Masahiko;Murata, Kazumasa;Yatou, Osamu;Nagato, Yasuo;Hirochika, Hirohiko

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为了便于水稻基因的功能分析,我们用内源反转录转座子Tos 17产生了约50,000个插入系。在田间对这些品系的M2代表型进行了观察,并根据53个表型描述符进行了表征。近一半的品系表现出一种以上的突变表型。最常见的表型是低生育力,其次是侏儒症。表型数据和每个品系的照片存储在Tos 17突变体小组基于网络的数据库中,该数据库具有水稻基因组中Tos 17插入点侧翼序列的数据集(http://tos.nias.affrc.go.jp/)。这种表型和侧翼序列数据的结合将刺激水稻基因的功能分析。
In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes.
DOI: 10.1104/pp.125.3.1248
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