Interspersed sequence organization and developmental representation of cloned poly(A) RNAs from sea urchin eggs.
Interspersed sequence organization and developmental representation of cloned poly(A) RNAs from sea urchin eggs.
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海胆卵中克隆的多聚 (A) RNA 的散布序列组织和发育表征。
DOI：
10.1016/s0022-2836(83)80340-4
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发表时间：
1983
影响因子：
5.6
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Posakony,JW;Flytzanis,CN;Britten,RJ;Davidson,EH
用两个克隆的基因组重复序列探针对构建的海胆卵母本Poly(A)RNA的随机引物互补DNA克隆文库进行了筛选。回收了与这些重复序列中的每一个反应的多组cDNA克隆。大多数克隆的转录本同时含有单拷贝和重复序列元件。除了共享的重复序列元件外，每组克隆的成员的重复区域和单拷贝区域都未能发生交叉反应。与克隆的母体RNA上的重复序列相连的单拷贝探针以不对称的方式表示。因此，给定的分散重复序列家族的许多不同的基因组成员在母体RNA中被表示。用几个重复探针进行的RNA凝胶杂交显示，大约10到20个显著的母体聚(A)RNAs包含每个重复序列家族的转录本。穿插的母体转录本的长度为3000到15,000个碱基。与来自克隆的cDNA的单拷贝探针反应的母体转录本在胚胎发育过程中持续存在，在某些情况下，类似的新合成的胚胎转录本似乎会增强。发现了两个在特定发育阶段出现不同长度的额外转录本的例子。不同个体海胆基因组中转录的单复制区具有高度的多态性，与近缘海胆物种的比较表明，在进化过程中，特定母体转录物的流行率和长度都发生了快速变化。出现在不同克隆转录本上的两个同源重复元件的核苷酸序列在每个可能的阅读框架中都显示了翻译终止密码子。这些重复序列显示出结构特征，表明在卵子发生过程中，存在着进化的转座到活跃的转录单位。重复元件及其侧翼的单复制区存在于很长的3‘或5’末端序列中，或者存在于母系聚(A)RNA中未经处理的插入序列中。这些发现导致我们提出这样的假设，即棘皮动物卵和早期胚胎中的大多数细胞质聚(A)RNA在形式上类似于出现在细胞核中的RNA，而不是后期细胞的信使RNA。
A random primed complementary DNA (cDNA) clone library constructed from total maternal poly(A) RNA of sea urchin eggs was screened with two cloned genomic repetitive sequence probes. Sets of cDNA clones reacting with each of these repetitive sequences were recovered. Most of the cloned transcripts included both single copy and repeat sequence elements. Except for the shared repeat sequence element, both the repetitive and single copy regions of the members of each set of clones failed to crossreact. Single copy probes linked to the repeats on the cloned maternal RNAs are represented in an asymmetric manner. It follows that many different genomic members of a given dispersed repeat sequence family are represented in the maternal RNA. RNA gel blots carried out with several repeat probes display about 10 to 20 prominent maternal poly(A) RNAs containing transcripts of each repetitive sequence family. The interspersed maternal transcripts are 3000 to 15,000 bases in length. Maternal transcripts reacting with single copy probes derived from the cloned cDNAs persist during embryonic development, and in some cases appear to be augmented by similar, newly synthesized embryo transcripts. Two examples were found in which additional transcripts of different length appear at specific developmental stages. The transcribed single copy regions are highly polymorphic in the genomes of different individual sea urchins, and comparisons of closely related sea urchin species showed that both the prevalence and length of specific maternal transcripts change rapidly during evolution. Nucleotide sequences of two homologous repeat elements occurring on different cloned transcripts displayed translation stop codons in every possible reading frame. These repeat sequences display structural features suggesting that there has been evolutionary transposition into transcription units active during oogenesis. The repeat elements and their flanking single copy regions reside either in very long 3′ or 5′-terminal sequences, or in unprocessed intervening sequences in the maternal poly(A) RNA. These findings lead us to the proposal that the majority of the cytoplasmic poly(A) RNA in echinoderm eggs and early embryos is similar in form to RNAs that occur in the nucleus rather than to the messenger RNA of later cells.
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影响因子：
64.8
作者：
POTTER, SS
通讯作者：
POTTER, SS
海胆胚胎中异质核RNA的分子类别。
影响因子：
5.6
作者：
L. Dubroff;M. Nemer
通讯作者：
M. Nemer
细胞质腺苷酸化和母体 RNA 的加工。
DOI：
10.1073/pnas.70.2.406
发表时间：
1973
影响因子：
11.1
作者：
I. Slater;D. Gillespie;D. W. Slater
通讯作者：
D. W. Slater
大多数海胆母体 mRNA 序列在每个丰度类别中都出现在聚腺苷酸化和非聚腺苷酸化分子中。
影响因子：
2.7
作者：
R. Duncan;T. Humphreys
通讯作者：
T. Humphreys
DOI：
10.1016/0045-6039(74)90020-7
发表时间：
1974
期刊：
Cell differentiation
影响因子：
--
作者：
G. Sconzo;I. Albanese;A. Rinaldi;G. F. LoPresti;G. Giudice
通讯作者：
G. Giudice