Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.
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DOI：
10.1371/journal.pone.0026444
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Alves PM
中科院分区：
文献类型：
--
作者：
Carinhas N;Robitaille AM;Moes S;Carrondo MJ;Jenoe P;Oliveira R;Alves PM
杆状病毒感染草地贪夜蛾细胞是一个系统的选择，以产生一系列的重组蛋白，疫苗，并可能，基因治疗载体。虽然杆状病毒基因组已被很好地表征，但S. frugiperda没有测序，并且病毒-宿主分子相互作用知之甚少。在此，我们描述了应用稳定同位素标记的氨基酸在细胞培养（SILAC），以获得第一个比较蛋白质组定量的S。frugiperda细胞生长过程中和早期杆状病毒感染。蛋白质组覆盖率最大化，通过编译一个搜索数据库与蛋白质注释从昆虫物种。感兴趣的差异蛋白质相关的能量代谢，内质网和氧化应激，但没有在杆状病毒感染的范围内进行研究。此外，在感染周期早期，关键病毒编码蛋白的表达减少，这表明与高细胞密度培养时病毒复制减少有关。这些发现对病毒学研究和基于杆状病毒的生物过程的改进具有意义。
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses.
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影响因子：
--
作者：
Carinhas N;Bernal V;Teixeira AP;Carrondo MJ;Alves PM;Oliveira R
通讯作者：
Oliveira R
影响因子：
3.7
作者：
CARSTENS, EB;TJIA, ST;DOERFLER, W
通讯作者：
DOERFLER, W
影响因子：
5.4
作者：
GRULA, MA;BULLER, PL;WEAVER, RF
通讯作者：
WEAVER, RF
影响因子：
5.3
作者：
Harvey, AJ;Bidwai, AP;Miller, LK
通讯作者：
Miller, LK
影响因子：
7
作者：
Mannova, Petra;Fang, Ruihua;Beretta, Laura
通讯作者：
Beretta, Laura