Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose.
Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose.
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DOI：
10.1016/j.dib.2018.05.140
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发表时间：
2018-08
期刊：
影响因子：
1.2
通讯作者：
Ward JM
中科院分区：
文献类型：
--
作者：
Bawn M;Subrizi F;Lye GJ;Sheppard TD;Hailes HC;Ward JM
本文中呈现的数据集与题为“One-pot，two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose”（Bawn et al. 2018年已出版）[1]。本文介绍了初步实验的数据，进行了调查新的耐热转酮酶（TK）的活动与l-阿拉伯糖。分析来自嗜热菌株的内部文库的转氨酶（TAm）序列，以比较与具有所需活性的表征TAm的同源性。DNA和氨基酸序列的所有酶的研究。TK和TAm反应产物的校准曲线也与各种一锅反应的色谱分析沿着提供。
The dataset presented in this article is related to the research article entitled “One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose” (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions.
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影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
14.9
作者：
The UniProt Consortium
通讯作者：
The UniProt Consortium
影响因子：
3.4
作者：
Bawn, Maria;Subrizi, Fabiana;Ward, John M.
通讯作者：
Ward, John M.
影响因子：
9.8
作者：
Subrizi, Fabiana;Cardenas-Fernandez, Max;Hailes, Helen C.
通讯作者：
Hailes, Helen C.
影响因子：
3.4
作者：
Cardenas-Fernandez, Max;Bawn, Maria;Lye, Gary J.
通讯作者：
Lye, Gary J.