Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase.
Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase.
复制标题
DOI：
10.1186/1471-2229-4-1
复制
发表时间：
2004-01-28
影响因子：
5.3
通讯作者：
Salt DE
中科院分区：
文献类型：
--
作者：
Ellis DR;Sors TG;Brunk DG;Albrecht C;Orser C;Lahner B;Wood KV;Harris HH;Pickering IJ;Salt DE
越来越明显的是，膳食硒在降低人类肺癌、结直肠癌和前列腺癌的发病率方面起着重要作用。不同形式的硒在其化学预防功效方面有所不同，硒-甲基硒代半胱氨酸是最有效的之一。有趣的是，硒积累植物双沟黄芪（双槽毒野豌豆）含有高达0.6%的其茎干重的硒-甲基硒代半胱氨酸。这种硒积累者生物合成硒-甲基硒代半胱氨酸的能力提供了一个关键的代谢分流，防止硒代半胱氨酸和硒代蛋氨酸进入蛋白质生物合成机器。这种代谢分流被认为对A.具双沟的利用这一机制在其他植物中可能提供了一个可能的途径，在植物中的硒耐受性的基因工程，理想的植物修复硒污染的土地。在这里，我们描述了一个硒代半胱氨酸甲基转移酶从A。bisulcatus在硒非积累植物拟南芥（塔勒cress）中工程化硒-甲基硒代半胱氨酸代谢。通过过度生产A。双沟酶硒代半胱氨酸甲基转移酶在A. thaliana中，我们已经引入了一种新的生物合成能力，该能力允许非积累者在芽中积累Se-甲基硒代半胱氨酸和γ-谷氨酰甲基硒代半胱氨酸。硒-甲基硒代半胱氨酸在A.拟南芥还赋予显著增加的亚硒酸盐耐受性和叶硒积累。这些结果证明了发展基于植物的硒-甲基硒代半胱氨酸的转基因生产以及植物抗亚硒酸盐生物工程的可行性。硒抗性是工程植物抗硒酸盐的第一步，硒酸盐是环境中硒的主要形式。
It has become increasingly evident that dietary Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Different forms of Se vary in their chemopreventative efficacy, with Se-methylselenocysteine being one of the most potent. Interestingly, the Se accumulating plant Astragalus bisulcatus (Two-grooved poison vetch) contains up to 0.6% of its shoot dry weight as Se-methylselenocysteine. The ability of this Se accumulator to biosynthesize Se-methylselenocysteine provides a critical metabolic shunt that prevents selenocysteine and selenomethionine from entering the protein biosynthetic machinery. Such a metabolic shunt has been proposed to be vital for Se tolerance in A. bisulcatus. Utilization of this mechanism in other plants may provide a possible avenue for the genetic engineering of Se tolerance in plants ideally suited for the phytoremediation of Se contaminated land. Here, we describe the overexpression of a selenocysteine methyltransferase from A. bisulcatus to engineer Se-methylselenocysteine metabolism in the Se non-accumulator Arabidopsis thaliana (Thale cress). By over producing the A. bisulcatus enzyme selenocysteine methyltransferase in A. thaliana, we have introduced a novel biosynthetic ability that allows the non-accumulator to accumulate Se-methylselenocysteine and γ-glutamylmethylselenocysteine in shoots. The biosynthesis of Se-methylselenocysteine in A. thaliana also confers significantly increased selenite tolerance and foliar Se accumulation. These results demonstrate the feasibility of developing transgenic plant-based production of Se-methylselenocysteine, as well as bioengineering selenite resistance in plants. Selenite resistance is the first step in engineering plants that are resistant to selenate, the predominant form of Se in the environment.
登录
查看更多内容
影响因子：
4.2
作者：
Fukushima, S;Takada, N;Ogawa, M
通讯作者：
Ogawa, M
影响因子：
4.7
作者：
IP, C;GANTHER, HE
通讯作者：
GANTHER, HE
影响因子：
6.1
作者：
Finley, JW;Ip, C;Whanger, PD
通讯作者：
Whanger, PD
影响因子：
6
作者：
Combs, GE
通讯作者：
Combs, GE
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF