Complex patterns of gene fission in the eukaryotic folate biosynthesis pathway.
Complex patterns of gene fission in the eukaryotic folate biosynthesis pathway.
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DOI：
10.1093/gbe/evu213
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发表时间：
2014-09-23
影响因子：
3.3
通讯作者：
Richards TA
中科院分区：
文献类型：
--
作者：
Maguire F;Henriquez FL;Leonard G;Dacks JB;Brown MW;Richards TA
共享的衍生基因组特征可用于极化系统发育关系，例如，基因融合已用于鉴定真核生物中的深分支关系。在这里，我们报告的进化分析的三个基因融合的folB，folK和folP，编码酶催化连续步骤从头叶酸的生物合成。在真核生物和原核生物的稀疏集合中发现了folK-folP融合。这表明一个古老的起源与一些基因丢失的真核生物可能作为一个结果，适应异养的生活方式。相比之下，folB-folK-folP基因特异于Amorphea分类群的镶嵌集合（一组包括：Amoebozoa、Apusomonadida、Breviatea和Opisthokonta）。接下来，我们研究了该特性的稳定性。我们确定了大量的基因丢失和总共9个基因裂变事件，或者是通过开放阅读框架的断裂（确定了4个事件），或者是通过组件结构域的丢失（确定了5个事件）。这表明这三个基因融合是高度不稳定的。这些数据与越来越多的数据相一致，这些数据表明基因裂变事件发生的相对速度很高。考虑到这些同源性的来源，我们的数据表明，folB-folK-folP基因融合存在于变形虫和后鞭毛纲动物的最后共同祖先中，但在后生动物包括人类基因组中不存在。这些基因的比较基因组数据提供了一个重要的资源，用于设计治疗策略，靶向从头叶酸生物合成途径的各种真核病原体，如卡氏阿米巴。
Shared derived genomic characters can be useful for polarizing phylogenetic relationships, for example, gene fusions have been used to identify deep-branching relationships in the eukaryotes. Here, we report the evolutionary analysis of a three-gene fusion of folB, folK, and folP, which encode enzymes that catalyze consecutive steps in de novo folate biosynthesis. The folK-folP fusion was found across the eukaryotes and a sparse collection of prokaryotes. This suggests an ancient derivation with a number of gene losses in the eukaryotes potentially as a consequence of adaptation to heterotrophic lifestyles. In contrast, the folB-folK-folP gene is specific to a mosaic collection of Amorphea taxa (a group encompassing: Amoebozoa, Apusomonadida, Breviatea, and Opisthokonta). Next, we investigated the stability of this character. We identified numerous gene losses and a total of nine gene fission events, either by break up of an open reading frame (four events identified) or loss of a component domain (five events identified). This indicates that this three gene fusion is highly labile. These data are consistent with a growing body of data indicating gene fission events occur at high relative rates. Accounting for these sources of homoplasy, our data suggest that the folB-folK-folP gene fusion was present in the last common ancestor of Amoebozoa and Opisthokonta but absent in the Metazoa including the human genome. Comparative genomic data of these genes provides an important resource for designing therapeutic strategies targeting the de novo folate biosynthesis pathway of a variety of eukaryotic pathogens such as Acanthamoeba castellanii.
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影响因子：
12.3
作者：
Dagan, Tal;Martin, William
通讯作者：
Martin, William
影响因子：
3.3
作者：
Cavalier-Smith, Thomas
通讯作者：
Cavalier-Smith, Thomas
影响因子：
4
作者：
Campbell, SA;Richards, TA;Roberts, CW
通讯作者：
Roberts, CW
DOI：
10.1098/rspb.2013.1755
发表时间：
2013-10-22
影响因子：
4.7
作者：
Brown, Matthew W.;Sharpe, Susan C.;Roger, Andrew J.
通讯作者：
Roger, Andrew J.
影响因子：
1.2
作者：
Andersson, Jan O.
通讯作者：
Andersson, Jan O.