Distinct associations of sputum and oral microbiota with atopic, immunologic, and clinical features in mild asthma.
Distinct associations of sputum and oral microbiota with atopic, immunologic, and clinical features in mild asthma.
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DOI：
10.1016/j.jaci.2020.03.028
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
National Heart, Lung, and Blood Institute’s ”AsthmaNet“
中科院分区：
文献类型：
--
作者：
Durack J;Christian LS;Nariya S;Gonzalez J;Bhakta NR;Ansel KM;Beigelman A;Castro M;Dyer AM;Israel E;Kraft M;Martin RJ;Mauger DT;Peters SP;Rosenberg SR;Sorkness CA;Wechsler ME;Wenzel SE;White SR;Lynch SV;Boushey HA;Huang YJ;National Heart, Lung, and Blood Institute’s ”AsthmaNet“
诱导痰或口腔样本的微生物组特征是否与成人特应性或轻度哮喘的特征有独特的关系尚不清楚。确定痰液和口腔微生物群与轻度特应性哮喘的临床或免疫学特征的关系，以及吸入性皮质类固醇（ICS）治疗对ICS初治哮喘受试者微生物群的影响。在一项多中心研究（NCT 01537133）中，分析了吸入氟替卡松治疗前后32名轻度特应性哮喘受试者、18名特应性非哮喘受试者和16名非特应性健康受试者的诱导痰和口腔冲洗液样本中的细菌微生物群特征。与临床和免疫学特征的关联进行了检查，包括特应性，2型炎症，免疫细胞群和细胞因子的标志物。痰菌负荷与支气管2型（T2）相关基因表达呈负相关。特定痰菌群的差异也与T2低哮喘表型相关，其中一个亚组显示肺部炎症介质升高和痰菌多样性降低。特定口腔微生物群的差异更能反映特应性状态。ICS治疗哮喘后，ICS无应答者的痰菌群组成结构比ICS应答者与基线的偏差更大。在这组有或没有ICS初治轻度哮喘的受试者中观察到痰液和口腔微生物群与免疫学特征的新关联。这些发现证实并扩展了我们先前的报告，即T2高哮喘受试者的支气管细菌负荷和组成复杂性降低，并额外鉴定了具有独特微生物群-免疫学关系的T2低亚组。在轻度特应性哮喘中，痰细菌微生物组特征与几种免疫学特征相关，包括2型-下气道炎症，并显示与吸入性皮质类固醇治疗反应相关的差异性变化。
Whether microbiome characteristics of induced sputum or oral samples demonstrate unique relationships to features of atopy or mild asthma in adults is unknown. To determine sputum and oral microbiota relationships to clinical or immunologic features in mild atopic asthma and the impact on the microbiota of inhaled corticosteroid (ICS) treatment administered to ICS-naïve asthmatic subjects. Bacterial microbiota profiles were analyzed in induced sputum and oral wash samples from 32 subjects with mild atopic asthma before and after inhaled fluticasone treatment, 18 atopic non-asthmatic subjects, and 16 non-atopic healthy subjects in a multicenter study (NCT01537133). Associations with clinical and immunologic features were examined, including markers of atopy, type 2 inflammation, immune cell populations and cytokines. Sputum bacterial burden inversely associated with bronchial expression of type 2 (T2)-related genes. Differences in specific sputum microbiota also associated with T2-low asthma phenotype, a subgroup of whom displayed elevations in lung inflammatory mediators and reduced sputum bacterial diversity. Differences in specific oral microbiota were more reflective of atopic status. After ICS treatment of asthmatics, the compositional structure of sputum microbiota showed greater deviation from baseline in ICS non-responders than in ICS-responders. Novel associations of sputum and oral microbiota to immunologic features were observed in this cohort of subjects with or without ICS-naïve mild asthma. These findings confirm and extend our prior report of reduced bronchial bacterial burden and compositional complexity in T2-high asthma subjects, with additional identification of a T2-low subgroup with a distinct microbiota-immunologic relationship. In mild atopic asthma, sputum bacterial microbiome characteristics associate with several immunologic features, including type 2-low airway inflammation, and display differential changes related to response to inhaled corticosteroid treatment.
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影响因子：
14.2
作者：
Marri, Pradeep Reddy;Stern, Debra A.;Wright, Anne L.;Billheimer, Dean;Martinez, Fernando D.
通讯作者：
Martinez, Fernando D.
影响因子：
4.4
作者：
Bhakta NR;Solberg OD;Nguyen CP;Nguyen CN;Arron JR;Fahy JV;Woodruff PG
通讯作者：
Woodruff PG
DOI：
10.4049/jimmunol.182.3.1404
发表时间：
2009-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kelly EA;Koziol-White CJ;Clay KJ;Liu LY;Bates ME;Bertics PJ;Jarjour NN
通讯作者：
Jarjour NN
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
DOI：
10.1164/rccm.201304-0775oc
发表时间：
2013-11-15
影响因子：
24.7
作者：
Goleva, Elena;Jackson, Leisa P.;Leung, Donald Y. M.
通讯作者：
Leung, Donald Y. M.