Asthma-associated differences in microbial composition of induced sputum.
Asthma-associated differences in microbial composition of induced sputum.
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DOI：
10.1016/j.jaci.2012.11.013
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发表时间：
2013-02
影响因子：
14.2
通讯作者：
Martinez, Fernando D.
中科院分区：
文献类型：
--
作者：
Marri, Pradeep Reddy;Stern, Debra A.;Wright, Anne L.;Billheimer, Dean;Martinez, Fernando D.
越来越明显的是，呼吸道的微生物定植可能在哮喘的发病机制中起作用。我们试图描述和比较哮喘和非哮喘成人诱导痰的微生物组。从10名非哮喘受试者和10名轻度活动性哮喘患者（8/10未使用吸入性皮质类固醇）中获得诱导痰样本。从痰液上清液中提取总DNA，并使用细菌16 s rRNA的V6高变区特异性引物进行扩增。将样品条形码化，并将等摩尔浓度的20个样品合并，并用454 GS FLX测序仪测序。通过与核糖体数据库计划中的16 s rRNA序列进行比较，将序列分配给细菌分类群。所有痰样品含有5个主要细菌门：厚壁菌门、变形菌门、放线菌门、梭杆菌门和拟杆菌门，其中前3门占总序列的90%以上。变形菌在哮喘患者中的比例较高（37%比15%，P <0.001）。相比之下，厚壁菌门（47%比63%，P = 0.17）和放线菌门（10%比14%，P = 0.36）在非哮喘受试者的样本中更常见，尽管这在统计学上并不显著。分层聚类产生2个显著聚类：一个主要包含哮喘样本，第二个主要包含非哮喘样本。此外，与非哮喘受试者样本相比，哮喘患者样本的细菌多样性更大。轻度哮喘患者呼吸道中的微生物组成发生改变，这与在更严重的哮喘患者中观察到的情况相似。
It is increasingly evident that microbial colonization of the respiratory tract might have a role in the pathogenesis of asthma. We sought to characterize and compare the microbiome of induced sputum in asthmatic and nonasthmatic adults. Induced sputum samples were obtained from 10 nonasthmatic subjects and 10 patients with mild active asthma (8/10 were not using inhaled corticosteroids). Total DNA was extracted from sputum supernatants and amplified by using primers specific for the V6 hypervariable region of bacterial 16s rRNA. Samples were barcoded, and equimolar concentrations of 20 samples were pooled and sequenced with the 454 GS FLX sequencer. Sequences were assigned to bacterial taxa by comparing them with 16s rRNA sequences in the Ribosomal Database Project. All sputum samples contained 5 major bacterial phyla: Firmicutes, Proteobacteria, Actinobacteria, Fusobacterium, and Bacteroidetes, with the first 3 phyla accounting for more than 90% of the total sequences. Proteobacteria were present in higher proportions in asthmatic patients (37% vs 15%, P < .001). In contrast, Firmicutes (47% vs 63%, P = .17) and Actinobacteria (10% vs 14%, P = .36) were found more frequently in samples from nonasthmatic subjects, although this was not statistically significant. Hierarchical clustering produced 2 significant clusters: one contained primarily asthmatic samples and the second contained primarily nonasthmatic samples. In addition, samples from asthmatic patients had greater bacterial diversity compared with samples from nonasthmatic subjects. Patients with mild asthma have an altered microbial composition in the respiratory tract that is similar to that observed in patients with more severe asthma.
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影响因子：
10
作者：
YAN, K;SALOME, C;WOOLCOCK, AJ
通讯作者：
WOOLCOCK, AJ
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
158.5
作者：
Bisgaard, Hans;Hermansen, Mette Northman;Pipper, Christian Bressen
通讯作者：
Pipper, Christian Bressen
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM