Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions.
Mapping and Validation of qHD7b: Major Heading-Date QTL Functions Mainly under Long-Day Conditions.
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qHD7b 的定位和验证：主要在长日照条件下的主要抽穗日期 QTL 功能
DOI：
10.3390/plants11172288
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发表时间：
2022-09-01
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抽穗期（HD）是影响成熟度、区域适应性和粮食产量的农艺性状之一。本研究是之前对三个群体进行的数量性状基因座 (QTL) 作图研究的后续研究，该研究发现了与 10 个农艺性状相关的总共 62 个 QTL。 7 号染色体上的 HD 的两个 QTL（qHD7a 和 qHD7b）具有共同的侧翼标记（RM3670），这可能是由于紧密连锁和/或统计方法的弱点所致。本研究的目的是在一组 76 个染色体片段替换系 (CSSL) 中绘制与 HD 相关的 QTL，使用 2997 株 BC5F2:3 植物精细绘制并验证其中一个 QTL (qHD7b)，并使用测序和表达分析鉴定候选基因。使用具有 120 个标记的 CSSL 基因分型并在两个短日照和两个长日照生长条件下进行评估，我们总共发现了 14 个 QTL（qHD2a、qHD4a、qHD4b、qHD5a、qHD6a、qHD6b、qHD7b、qHD7c、qHD8a、qHD10a、qHD10b、qHD11a、 qHD12a 和 qHD12b)。然而，在所有四种环境中，只有 qHD6a 和 qHD7b 被一致检测到。 qHD6a 和 qHD7b 解释的表型方差分别为 10.1% 至 36.1%（平均 23.1%）和 8.1% 至 32.8%（平均 20.5%）。其中一个 CSSL 品系 (CSSL52) 在 qHD7b 基因座上包含来自早穗谢庆枣 B (XQZB) 亲本的片段，然后用于开发 BC5F2:3 群体以进行精细作图和验证。使用在不同日长和温度下评估四个季节的回交群体，qHD7b 区间被界定为 912.7-kb 区域，位于 RM5436 和 RM5499 之间。测序和表达分析共发现29个候选基因，其中Ghd7（Os07g0261200）是影响水稻抽穗期、株高和籽粒产量的知名基因。通过 CRISPR/Cas9 基因编辑产生的 ghd7 突变体表现出早期抽穗现象。综上所述，之前和当前研究的结果揭示了7号染色体上抽穗日期一致的QTL，它不仅与已知基因的物理位置一致，而且与控制水稻柱头发挥率和小穗数量的两个主要效应QTL一致。这些结果为标记辅助育种更广泛的适应性以开发高产水稻品种做出了贡献。
Heading date (HD) is one of the agronomic traits that influence maturity, regional adaptability, and grain yield. The present study was a follow-up of a previous quantitative trait loci (QTL) mapping study conducted on three populations, which uncovered a total of 62 QTLs associated with 10 agronomic traits. Two of the QTLs for HD on chromosome 7 (qHD7a and qHD7b) had a common flanking marker (RM3670) that may be due to tight linkage, and/or weakness of the statistical method. The objectives of the present study were to map QTLs associated with HD in a set of 76 chromosome segment substitution lines (CSSLs), fine map and validate one of the QTLs (qHD7b) using 2997 BC5F2:3 plants, and identify candidate genes using sequencing and expression analysis. Using the CSSLs genotyped with 120 markers and evaluated under two short-day and two long-day growing conditions, we uncovered a total of fourteen QTLs (qHD2a, qHD4a, qHD4b, qHD5a, qHD6a, qHD6b, qHD7b, qHD7c, qHD8a, qHD10a, qHD10b, qHD11a, qHD12a, and qHD12b). However, only qHD6a and qHD7b were consistently detected in all four environments. The phenotypic variance explained by qHD6a and qHD7b varied from 10.1% to 36.1% (mean 23.1%) and from 8.1% to 32.8% (mean 20.5%), respectively. One of the CSSL lines (CSSL52), which harbored a segment from the early heading XieqingzaoB (XQZB) parent at the qHD7b locus, was then used to develop a BC5F2:3 population for fine mapping and validation. Using a backcross population evaluated for four seasons under different day lengths and temperatures, the qHD7b interval was delimited to a 912.7-kb region, which is located between RM5436 and RM5499. Sequencing and expression analysis revealed a total of 29 candidate genes, of which Ghd7 (Os07g0261200) is a well-known gene that affects heading date, plant height, and grain yield in rice. The ghd7 mutants generated through CRISPR/Cas9 gene editing exhibited early heading. Taken together, the results from both the previous and present study revealed a consistent QTL for heading date on chromosome 7, which coincided not only with the physical position of a known gene, but also with two major effect QTLs that controlled the stigma exertion rate and the number of spikelets in rice. The results provide contributions to the broader adaptability of marker-assisted breeding to develop high-yield rice varieties.
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影响因子：
5.3
作者：
Hori K;Nonoue Y;Ono N;Shibaya T;Ebana K;Matsubara K;Ogiso-Tanaka E;Tanabata T;Sugimoto K;Taguchi-Shiobara F;Yonemaru J;Mizobuchi R;Uga Y;Fukuda A;Ueda T;Yamamoto S;Yamanouchi U;Takai T;Ikka T;Kondo K;Hoshino T;Yamamoto E;Adachi S;Nagasaki H;Shomura A;Shimizu T;Kono I;Ito S;Mizubayashi T;Kitazawa N;Nagata K;Ando T;Fukuoka S;Yamamoto T;Yano M
通讯作者：
Yano M
影响因子：
6.6
作者：
Alvarado, Gregorio;Rodriguez, Francisco M.;Lopez-Cruz, Marco A.
通讯作者：
Lopez-Cruz, Marco A.
qHD1b 的精细定位和候选基因分析，qHD1b 是通过上调 Ehd1 促进普通野生稻 (Oryza rufipogon) 开花的 QTL
影响因子：
6.6
作者：
Liu, Ling;Zhang, Yingxin;Wu, Weixun
通讯作者：
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影响因子：
2.4
作者：
Bessho-Uehara K;Furuta T;Masuda K;Yamada S;Angeles-Shim RB;Ashikari M;Takashi T
通讯作者：
Takashi T
影响因子：
4.9
作者：
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通讯作者：
Yano, Masahiro