Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration.
Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration.
复制标题
ECM和微环境特性在小胶质细胞BV2细胞迁移上的微流体伤口愈合测定。
DOI：
10.3390/bios13020290
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发表时间：
2023-02-17
期刊：
影响因子：
5.4
通讯作者：
Packirisamy, Muthukumaran
中科院分区：
文献类型：
--
作者：
Moghadam, Ehsan Yazdanpanah;Sonenberg, Nahum;Packirisamy, Muthukumaran
关键词：
小胶质细胞作为中枢神经系统（CNS）的常驻免疫细胞，在发育和病理生理条件下具有高度运动性和迁移性。在迁移过程中，小胶质细胞根据大脑的各种物理和化学特性与周围环境相互作用。本研究开发了一种微流控伤口愈合芯片，用于研究小胶质BV2细胞在包被细胞外基质（ecm）的基质和通常用于生物应用的细胞迁移基质上的迁移。为了产生无细胞空间（伤口），利用重力作为驱动力使胰蛋白酶随装置流动。结果表明，尽管有划痕实验，但使用微流体实验，在不去除细胞外基质涂层（纤维连接蛋白）的情况下，创建了无细胞区域。结果发现，包被聚l -赖氨酸（PLL）和明胶的底物刺激了小胶质细胞BV2的迁移，而包被胶原蛋白和纤维连接蛋白的底物与对照组（未包被的玻璃底物）相比，具有抑制作用。此外，结果表明，聚苯乙烯衬底诱导的细胞迁移率高于PDMS和玻璃衬底。微流控迁移实验为进一步了解小胶质细胞在大脑中的迁移机制提供了一个更接近体内条件的体外微环境，其中环境特性在稳态和病理条件下发生变化。
Microglia cells, as the resident immune cells of the central nervous system (CNS), are highly motile and migratory in development and pathophysiological conditions. During their migration, microglia cells interact with their surroundings based on the various physical and chemical properties in the brain. Herein, a microfluidic wound-healing chip is developed to investigate microglial BV2 cell migration on the substrates coated with extracellular matrixes (ECMs) and substrates usually used for bio-applications on cell migration. In order to generate the cell-free space (wound), gravity was utilized as a driving force to flow the trypsin with the device. It was shown that, despite the scratch assay, the cell-free area was created without removing the extracellular matrix coating (fibronectin) using the microfluidic assay. It was found that the substrates coated with Poly-L-Lysine (PLL) and gelatin stimulated microglial BV2 migration, while collagen and fibronectin coatings had an inhibitory effect compared to the control conditions (uncoated glass substrate). In addition, the results showed that the polystyrene substrate induced higher cell migration than the PDMS and glass substrates. The microfluidic migration assay provides an in vitro microenvironment closer to in vivo conditions for further understanding the microglia migration mechanism in the brain, where the environment properties change under homeostatic and pathological conditions.
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影响因子：
5.7
作者：
Murphy MC;Huston J 3rd;Ehman RL
通讯作者：
Ehman RL
影响因子：
4.2
作者：
Hiscox LV;Johnson CL;McGarry MDJ;Perrins M;Littlejohn A;van Beek EJR;Roberts N;Starr JM
通讯作者：
Starr JM
影响因子：
5.3
作者：
Blaschke SJ;Demir S;König A;Abraham JA;Vay SU;Rabenstein M;Olschewski DN;Hoffmann C;Hoffmann M;Hersch N;Merkel R;Hoffmann B;Schroeter M;Fink GR;Rueger MA
通讯作者：
Rueger MA
影响因子：
3.4
作者：
Chauvet, D.;Imbault, M.;Tanter, M.
通讯作者：
Tanter, M.
影响因子：
3
作者：
Chen, Chien-Chang;Hsieh, Patrick Chin-Ho;Yeh, Ming-Long
通讯作者：
Yeh, Ming-Long