100 Days of marine Synechococcus-Ruegeria pomeroyi interaction: A detailed analysis of the exoproteome.
100 Days of marine Synechococcus-Ruegeria pomeroyi interaction: A detailed analysis of the exoproteome.
复制标题
DOI：
10.1111/1462-2920.14012
复制
发表时间：
2018-03
影响因子：
5.1
通讯作者：
Christie-Oleza JA
中科院分区：
文献类型：
--
作者：
Kaur A;Hernandez-Fernaud JR;Aguilo-Ferretjans MDM;Wellington EM;Christie-Oleza JA
海洋光养和异养相互作用对于维持海洋营养平衡至关重要，因为必需营养物质在沉入无光层之前需要快速循环。这项研究的目的是强调驱动这些相互作用的分子机制。为此，我们对海洋微微蓝杆菌聚球蓝藻模型之间 100 天的共培养进行了详细的外蛋白质组时间过程分析。 WH7803 和玫瑰杆菌菌株 Ruegeria pomeroyi DSS-3，均存在于营养丰富的天然贫营养海水中。蛋白质组数据显示，在异养生物的情况下，初始生长阶段和稳定状态阶段之间的转变是由有机质可用性引起的运动性转变引起的。光养生物通过减少选择性渗漏、增加必需营养物质的获取和分泌功能未知的保守蛋白来适应海水寡营养。我们还报道了聚球藻产生的细胞外超氧化物歧化酶的丰度惊人地高，以及异养生物用来裂解有机基团和水解蓝藻产生的聚合有机物的潜在水解酶候选物的动态分泌。我们在此提供的时间进程数据集将成为理解支撑海洋光养-异养相互作用的分子过程的参考。
Marine phototroph and heterotroph interactions are vital in maintaining the nutrient balance in the oceans as essential nutrients need to be rapidly cycled before sinking to aphotic layers. The aim of this study was to highlight the molecular mechanisms that drive these interactions. For this, we generated a detailed exoproteomic time‐course analysis of a 100‐day co‐culture between the model marine picocyanobacterium Synechococcus sp. WH7803 and the Roseobacter strain Ruegeria pomeroyi DSS‐3, both in nutrient‐enriched and natural oligotrophic seawater. The proteomic data showed a transition between the initial growth phase and stable‐state phase that, in the case of the heterotroph, was caused by a switch in motility attributed to organic matter availability. The phototroph adapted to seawater oligotrophy by reducing its selective leakiness, increasing the acquisition of essential nutrients and secreting conserved proteins of unknown function. We also report a surprisingly high abundance of extracellular superoxide dismutase produced by Synechococcus and a dynamic secretion of potential hydrolytic enzyme candidates used by the heterotroph to cleave organic groups and hydrolase polymeric organic matter produced by the cyanobacterium. The time course dataset we present here will become a reference for understanding the molecular processes underpinning marine phototroph‐heterotroph interactions.
登录
查看更多内容
影响因子：
28.3
作者：
Christie-Oleza JA;Sousoni D;Lloyd M;Armengaud J;Scanlan DJ
通讯作者：
Scanlan DJ
影响因子：
6.4
作者：
Bryson S;Li Z;Pett-Ridge J;Hettich RL;Mayali X;Pan C;Mueller RS
通讯作者：
Mueller RS
影响因子：
6.8
作者：
Hehemann, Jan-Hendrik;Boraston, Alisdair B.;Czjzek, Mirjam
通讯作者：
Czjzek, Mirjam
影响因子：
11
作者：
D'Ambrosio, Lindsay;Ziervogel, Kai;Arnosti, Carol
通讯作者：
Arnosti, Carol
影响因子：
5.1
作者：
Christie-Oleza JA;Armengaud J;Guerin P;Scanlan DJ
通讯作者：
Scanlan DJ