Labilibaculum antarcticum sp. nov., a novel facultative anaerobic, psychrotorelant bacterium isolated from marine sediment of Antarctica
Labilibaculum antarcticum sp. nov., a novel facultative anaerobic, psychrotorelant bacterium isolated from marine sediment of Antarctica
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南极唇杆菌 sp.
DOI：
10.1007/s10482-019-01345-w
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Fukui Manabu
中科院分区：
文献类型：
--
作者：
Watanabe Miho;Kojima Hisaya;Fukui Manabu
从南极海洋沉积物中分离到一株兼性厌氧兼性嗜冷菌，命名为SPP2T。分离到的细胞呈长棒状(0.5 × 5-10μm)，革兰氏染色阴性，具有滑行能力。生长的最适氯化钠浓度为2~3%，最适温度为18~22℃。在乳酸盐存在下，菌株SPP2T不能利用硫酸盐和硝酸盐作为电子受体。测得基因组DNA的G+C含量为36.0mmol%。细胞内主要脂肪酸组成为Anteiso-C15：0和iso-C15：0。MK-7是主要的呼吸对苯二酚。基于16S rRNA基因的系统发育分析表明，该新菌株属于Marinifaceae，与还原曼氏唇杆菌59.10~(-2)MT有较近的亲缘关系，16S rRNA基因序列同源性达98%。SPP2T株与其近缘株基因组序列的同源性分别为84%和27.3%，均低于物种划分的阈值。在系统发育和表型特征分析的基础上，发现北极唇状杆菌Labilibaculum antarcticumsp.11月模式菌株SPP2T(= NBRC111151T= CECT9460T)。
A novel facultative anaerobic and facultative psychrophilic bacterium, designated SPP2T, was isolated from an Antarctic marine sediment. Cells of the isolate were observed to be long rods (0.5 × 5–10 μm), Gram-stain negative and to have gliding motility. For growth, the optimum NaCl concentration was found to be 2–3% and the optimum temperature to be 18–22 °C. Strain SPP2Tcannot use sulfate and nitrate as electron acceptors in the presence of lactate. The G+C content of the genomic DNA was determined to be 36.0 mol%.. The major cellular fatty acids were identified as anteiso-C15:0and iso-C15:0. MK-7 was found to be the predominant respiratory quinone. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belongs to the familyMarinifilaceaeand to be closely related toLabilibaculum manganireducens59.10-2MTwith 16S rRNA gene sequence identity of 98%. The OrthoANI and dDDH values between the genome sequences of strain SPP2Tand its close relative were 84% and 27.3%, which are lower than the threshold values for species delineation. On the basis of phylogenetic and phenotypic characterisation,Labilibaculum antarcticumsp. nov. is proposed with the type strain SPP2T(= NBRC 111151T= CECT 9460T).
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影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
5.2
作者：
Vandieken V;Marshall IPG;Niemann H;Engelen B;Cypionka H
通讯作者：
Cypionka H
Marinisporobacter balticus gen nov , sp nov , Desulfosporosinus nitroreducens sp nov 和 Desulfosporosinus fructosivorans sp nov , 从波罗的海地下沉积物中分离出的新孢子形成细菌
DOI：
10.1099/ijsem.0.001883
发表时间：
2017
影响因子：
2.8
作者：
Vandieken V;Niemann H;Engelen B;Cypionka H
通讯作者：
Cypionka H
DOI：
10.1099/ijs.0.009027-0
发表时间：
2009-09-01
影响因子：
2.8
作者：
Na, Hyunsoo;Kim, Seil;Chun, Jongsik
通讯作者：
Chun, Jongsik