Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea.
Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea.
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Marinisporobacter balticus gen nov , sp nov , Desulfosporosinus nitroreducens sp nov 和 Desulfosporosinus fructosivorans sp nov , 从波罗的海地下沉积物中分离出的新孢子形成细菌
DOI：
10.1099/ijsem.0.001883
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发表时间：
2017
影响因子：
2.8
通讯作者：
Cypionka H
中科院分区：
文献类型：
--
作者：
Vandieken V;Niemann H;Engelen B;Cypionka H
从国际海洋发现计划347次波罗的海探险期间采集的海底沉积物中分离到四种革兰氏阳性内孢子菌。1株(59.4MT)专性异养菌通过好氧呼吸和厌氧发酵生长。最适生长条件为：0.5 %NaC l，25 °C，pH7.0-7.3。经16S rRNA基因序列分析，与金黄色葡萄球菌( %同源性为92.3%)、铁还原假丝孢杆菌(92.2 %)、亚地芽孢杆菌(91.9% %)和肽发酵嗜碱性杆菌(91.7 %)的16S rRNA基因序列具有较高的同源性。根据表型分析和基因分型的结果，我们认为59.4MTre菌株是一个新属--巴氏滨海孢杆菌属的一个新种。Nov.，sp.11月，模式菌株59.4MT(=DSM 102940T=JCM 31103T)。另有3株菌株59.4F、59.4BT和63.6FT与硫磺芽孢杆菌属(Desulfosporinus)有亲缘关系，严格以厌氧硫酸盐还原菌的形式生长。这些菌株还使用硫代硫酸盐、元素硫、亚硫酸盐和DMSO作为电子受体，氢气作为电子供体。菌株59.4F和59.4BT的16S rRNA基因序列完全相同，与乳链球菌(97.8 %)、链球菌属(97.3 %)和东方链球菌属(97.3 %)的16S rRNA基因序列相似性最高。菌株63.6FT与TOD亲缘关系密切。革兰氏阴性杆菌(97.7 %)、子午沙门氏菌(96.6 %)和革兰氏阴性杆菌(96.6%)。HIPPEI(96.5 %)。菌株59.4BT和63.6FT的16SRRNA基因序列相似性为96.6 %。我们建议将其命名为Desulfosporosinus Nitrative Reducenssp.和模式菌株59.4BT(=DSM 101608T=JCM 31105T)和果糖豆腐霉菌Desulfosporosinus fuctosivoranssp.11月，模式菌株63.6FT(=DSM 101609T=JCM 31106T)。
Four novel Gram-stain-positive, endospore-forming bacteria of the orderClostridialeswere isolated from subsurface sediments sampled during International Ocean Discovery Program Expedition 347 to the Baltic Sea. One strain (59.4MT) grew as an obligate heterotroph by aerobic respiration and anaerobically by fermentation. Optimum growth was observed with 0.5 % NaCl at 25 °C and pH 7.0–7.3. Analysis of 16S rRNA gene sequences of 59.4MTrevealedAlkaliphilus transvaalensis(92.3 % identity),CandidatusGeosporobacter ferrireducens (92.2 %),Geosporobacter subterraneus(91.9 %) andAlkaliphilus peptidifermentans(91.7 %) to be the closest relatives. On the basis of the results of phenotypic and genotypic analyses, we propose that strain 59.4MTrepresents a novel species within a novel genus,Marinisporobacter balticusgen. nov., sp. nov., with the type strain 59.4MT(=DSM 102940T=JCM 31103T). Three other strains, 59.4F, 59.4BTand 63.6FT, were affiliated with the genusDesulfosporosinusand grew as strictly anaerobic sulfate reducers. These strains additionally used thiosulfate, elemental sulfur, sulfite and DMSO as electron acceptors and hydrogen as an electron donor. Strains 59.4F and 59.4BThad identical 16S rRNA gene sequences, which were most similar to those ofDesulfosporosinus lacus(97.8 %),Desulfosporosinus hippei(97.3 %) andDesulfosporosinus orientis(97.3 %). Strain 63.6FTwas closely related toD. lacus(97.7 %), Desulfosporosinus meridiei(96.6 %) andD. hippei(96.5 %). The similarity of 16S rRNA gene sequences of strains 59.4BTand 63.6FTwas 96.6 %. We propose the new namesDesulfosporosinus nitroreducenssp. nov., incorporating strain 59.4F (=DSM 101562=JCM 31104) and the type strain 59.4BT(=DSM 101608T=JCM 31105T), andDesulfosporosinus fructosivoranssp. nov., with the type strain 63.6FT(=DSM 101609T=JCM 31106T).
DOI：
10.1099/ijs.0.64058-0
发表时间：
2006-04-01
影响因子：
2.8
作者：
Vandieken, V;Knoblauch, C;Jorgensen, BB
通讯作者：
Jorgensen, BB
影响因子：
2.8
作者：
TSCHECH, A;PFENNIG, N
通讯作者：
PFENNIG, N
DOI：
10.1099/ijs.0.035238-0
发表时间：
2013
影响因子：
2.8
作者：
Bruno Mayeux;M. Fardeau;Manon Bartoli;L. Casalot;A. Vinsot;M. Labat
通讯作者：
M. Labat