New insight into ovary abortion during ovary development of hazelnut through a combined proteomic and transcriptomic analysis
New insight into ovary abortion during ovary development of hazelnut through a combined proteomic and transcriptomic analysis
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通过蛋白质组学和转录组学的联合分析，对榛子卵巢发育过程中卵巢流产的新认识
DOI：
10.1016/j.scienta.2018.02.003
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发表时间：
2018-04
影响因子：
4.3
通讯作者：
Cheng Yunqing
中科院分区：
文献类型：
--
作者：
Liu Jianfeng;Xing Jiyang;Fang Jun;Ai Pengfei;Cheng Yunqing
榛（Corylusspp.）是桦木科最重要的经济树种。受精期频繁的子房败育导致雌蕊花掉落率高，果簇果实数少，造成产量损失。为了更好地了解榛子卵巢流产的形成机制，我们进行了转录组学和蛋白质组学分析，并确定了响应卵巢流产的基因和蛋白质。利用RNA-seq和iTRAQ技术分别鉴定了89,846个单基因和4343个蛋白。其中，在发育卵巢和败育卵巢中分别有1895个单基因和710个蛋白的差异表达和积累。此外，我们使用京都基因和基因组百科全书（KEGG）分析，揭示了转录组学和蛋白质组学分析中显著富集的途径。这些包括苯丙氨酸代谢途径，苯丙素的生物合成，次生代谢物的生物合成，以及抗坏血酸和醛酸盐的代谢。转录组-蛋白质组整合分析显示42个相关性。其中32个变化趋势相同的相关基因（涉及代谢应激反应、维管链发育、水分运输和种子发育）可能与子房的形成有关。重要的是，乙烯（ETH）、茉莉酸（JA）和水杨酸（SA）信号转导通路可能通过上调乙烯反应性转录因子1（ERF1/2）、茉莉酸ZIM结构域蛋白（JAZ）、转录因子MYC2 （MYC2）、转录因子TGA和发病相关蛋白1（PR-1）参与了流产卵巢形成的调控。我们的工作将有助于鉴定卵巢败育相关基因和蛋白，并为了解果实发育的分子机制提供新的思路。
Hazel (Corylusspp.) is the most economically important species in the Betulaceae family. Frequent ovary abortion during the prefertilization stage causes the high drop ratio of pistillate flowers and smaller fruit numbers in a fruit cluster, resulting in yield loss. To better understand the mechanism of ovary abortion formation in hazel, we conducted transcriptomic and proteomic analyses and identified genes and proteins that were altered in response to ovary abortion. A total of 89,846 unigenes and 4343 proteins were identified using RNA-seq and iTRAQ technology, respectively. Among them, 1895 unigenes and 710 proteins were differentially expressed and accumulated in the developing and abortive ovary respectively. Furthermore, we employed the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, revealing pathways that are significantly enriched in both transcriptomic and proteomic assays. These include the phenylalanine metabolic pathway, biosynthesis of phenylpropanoid, biosynthesis of secondary metabolites, and ascorbate and aldarate metabolism. Transcriptome-proteome integrative analysis revealed 42 correlations. Among these, 32 correlations with same changing trend (that are involved in metabolism stress response, vascular strand development, water transport, and seed development) are likely associated with abortive ovary formation. Importantly, ethylene (ETH), jasmonic acid (JA), and salicylic acid (SA) signal transduction pathways may contribute to the regulation of the abortive ovary formation via the up-regulation of ethylene-responsive transcription factor 1 (ERF1/2), jasmonate ZIM domain-containing protein (JAZ), transcription factor MYC2 (MYC2), transcription factor (TGA), and pathogenesis-related protein 1(PR-1). Our work will facilitate the identification of ovary abortion related genes and proteins and provide insights into the molecular mechanism of fruit development.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
发育和败育榛子胚珠植物激素生物合成和信号转导途径的比较
影响因子：
4.2
作者：
Cheng, Yunqing;Zhao, Yixin;Ming, Yuetong
通讯作者：
Ming, Yuetong
榛子果实发育过程中二钠荧光素转运的时间变化
影响因子：
4.3
作者：
Liu, Jianfeng;Cheng, Yunqing;Wang, Zhanwu
通讯作者：
Wang, Zhanwu
DOI：
10.1007/978-1-59745-198-7_4
发表时间：
2009-01-01
期刊：
PROTEIN PROTOCOLS HANDBOOK, THIRD EDITION
影响因子：
--
作者：
Kruger, Nicholas J.
通讯作者：
Kruger, Nicholas J.