Characterization of the pColV‐K30 encoded cloacin DF13/aerobactin outer membrane receptor protein of Escherichia coli; isolation and purification of the protein and analysis of its nucleotide sequence and primary structure
Characterization of the pColV‐K30 encoded cloacin DF13/aerobactin outer membrane receptor protein of Escherichia coli; isolation and purification of the protein and analysis of its nucleotide sequence and primary structure
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大肠杆菌pColV-K30编码的cloacin DF13/aerobactin外膜受体蛋白的表征及其核苷酸序列和一级结构分析；
DOI：
10.1111/j.1574-6968.1985.tb01583.x
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发表时间：
1985
影响因子：
2.1
通讯作者：
B. Oudega
中科院分区：
文献类型：
--
作者：
W. Krone;F. Stegehuis;G. Koningstein;C. V. Doorn;B. Roosendaal;F. K. Graaf;B. Oudega
摘要通过重复Triton X-100提取和亲和层析，从大肠杆菌(PFS8)和爱德华克雷伯氏菌(Klebsiella Edwardsii)中分离和纯化了氯沙星DF13/需氧肌动蛋白受体蛋白。两种受体蛋白在SDS-PAGE上均呈单一蛋白条带。表观MR值分别为74000和76000。测定了纯化的大肠杆菌受体蛋白(PFS8)与爱德华克雷伯氏菌受体蛋白和克罗沙星受体蛋白DF13的结合常数。分别为2.0×10 8M−1和1.0×10 9M−1。测定了pFS8上编码Cloacin DF13/Aerobactin受体蛋白的pColV-K30基因的核苷酸序列，推测了该蛋白的一级结构和二级结构。结果表明，pColV-K30特异性受体蛋白可能是作为前体合成的，信号序列为25个氨基酸残基。成熟蛋白的相对分子质量为77 345。
Abstract The cloacin DF13/aerobactin receptor protein from Escherichia coli (pFS8) and from Klebsiella edwardsii were isolated by repeated Triton X-100 extractions and purified by affinity chromatography. Both receptor proteins ran as a single protein band on SDS-PAGE. Their apparent M r values were 74 000 and 76 000, respectively. The binding constants of the purified receptor proteins from E. coli (pFS8) and K. edwardsii and cloacin DF13 were determined. Values of 2.0 × 10 8 M −1 and 1.0 × 10 9 M −1 , respectively, were found. The nucleotide sequence of the pColV-K30 gene, contained on pFS8 and encoding the cloacin DF13/aerobactin receptor protein, was determined and the primary structure of the protein as well as its secondary structure were deduced. The results revealed that the pColV-K30-specified receptor protein might be synthesized as a precursor, with a signal sequence of 25 amino acid residues. The mature protein has an M r of 77 345.
大肠杆菌 K12 中铁肠杆菌素外膜受体的特性和蛋白水解。
影响因子：
2.9
作者：
Fiss,EH;Stanley-Samuelson,P;Neilands,JB
通讯作者：
Neilands,JB
大肠杆菌主要外膜蛋白的氨基酸序列同源性。
DOI：
10.1073/pnas.81.4.1048
发表时间：
1984
影响因子：
11.1
作者：
Nikaido,H;Wu,HC
通讯作者：
Wu,HC