Investigating supramolecular systems using Förster resonance energy transfer.

Investigating supramolecular systems using Förster resonance energy transfer.
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DOI:
10.1039/c8cs00278a
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发表时间:
2018-09-17
影响因子:
46.2
通讯作者:
Mulder WJM
Mulder WJM
中科院分区:
化学1区
文献类型:
--
作者:
Teunissen AJP ;Pérez-Medina C ;Meijerink A ;Mulder WJM

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超分子体系在材料科学、生物化学、分析化学和纳米医学等领域有着广泛的应用。然而,由于发生非共价现象的时间尺度、结合强度、距离和浓度的范围很广,分析这类系统可能是具有挑战性的。由于它们的通用性和敏感性,基于Förster共振能量转移(FRET)的技术非常适合于应对这些挑战。在这里,我们详细介绍了FRET被用来研究合成和生物超分子体系中的非共价相互作用的方法。在其他主题中,我们检查了测量分子作用力、确定蛋白质构象、监控组装动力学以及从纳米颗粒中可视化体内药物释放的方法。此外,我们强调了多重FRET技术,讨论了该领域的局限性,并提供了对新发展的看法。本文详细介绍了Förster共振能量转移(FRET)用于研究天然和合成超分子体系的方法。
Supramolecular systems have applications in areas as diverse as materials science, biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems can be challenging due to the wide range of time scales, binding strengths, distances, and concentrations at which non-covalent phenomena take place. Due to their versatility and sensitivity, Förster resonance energy transfer (FRET)-based techniques are excellently suited to meet such challenges. Here, we detail the ways in which FRET has been used to study non-covalent interactions in both synthetic and biological supramolecular systems. Among other topics, we examine methods to measure molecular forces, determine protein conformations, monitor assembly kinetics, and visualize in vivo drug release from nanoparticles. Furthermore, we highlight multiplex FRET techniques, discuss the field’s limitations, and provide a perspective on new developments. This review details the ways Förster resonance energy transfer (FRET) can be used to study natural and synthetic supramolecular systems.
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