Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons.
Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons.
复制标题
DOI：
10.1186/1471-2202-7-17
复制
发表时间：
2006-02-17
期刊：
影响因子：
2.4
通讯作者：
Bloch LM
中科院分区：
文献类型：
--
作者：
Zhong J;Zhang T;Bloch LM
信使RNA的靶向转运和突触附近的局部蛋白质合成对突触可塑性很重要。为了了解树突状细胞mRNA库的组成，我们从大鼠海马CA1区解剖出放射层（树突状层），并利用一组cDNA微阵列比较了其mRNA含量与细胞体层（细胞体层）的mRNA含量。在树突部分中具有过度表达的RNA被注释并分类到功能组中。我们已经确定了154个树突状mRNA的候选人，这可以安排到受体和通道，信号分子，细胞骨架和粘附分子，并参与膜运输，蛋白质合成，翻译后蛋白质修饰和蛋白质降解的因素的类别。从我们的筛选中鉴定出先前已知的树突状mRNA，如MAP 2、钙调蛋白和G蛋白γ亚基，以及编码已知对突触可塑性和记忆重要的蛋白质的mRNA，如spinophilin、Pumilio、eEF1A和MHC I类分子。此外，在树突中还发现了编码核糖体蛋白的mRNA。我们的研究结果表明，神经元运输各种mRNA到树突，不仅直接参与调节突触可塑性，而且其他人在细胞代谢中发挥更常见的作用。
Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. RNAs that have over-representation in the dendritic fraction were annotated and sorted into function groups. We have identified 154 dendritic mRNA candidates, which can be arranged into the categories of receptors and channels, signaling molecules, cytoskeleton and adhesion molecules, and factors that are involved in membrane trafficking, in protein synthesis, in posttranslational protein modification, and in protein degradation. Previously known dendritic mRNAs such as MAP2, calmodulin, and G protein gamma subunit were identified from our screening, as were mRNAs that encode proteins known to be important for synaptic plasticity and memory, such as spinophilin, Pumilio, eEF1A, and MHC class I molecules. Furthermore, mRNAs coding for ribosomal proteins were also found in dendrites. Our results suggest that neurons transport a variety of mRNAs to dendrites, not only those directly involved in modulating synaptic plasticity, but also others that play more common roles in cellular metabolism.
登录
查看更多内容
影响因子：
16.2
作者：
Kanai, Y;Dohmae, N;Hirokawa, N
通讯作者：
Hirokawa, N
影响因子：
5.3
作者：
Huang, F;Chotiner, JK;Steward, O
通讯作者：
Steward, O
影响因子：
56.9
作者：
Huh, GS;Boulanger, LM;Shatz, CJ
通讯作者：
Shatz, CJ
DOI：
10.1073/pnas.97.16.9287
发表时间：
2000-08-01
影响因子：
11.1
作者：
Feng, J;Yan, Z;Greengard, P
通讯作者：
Greengard, P
DOI：
10.1006/bbrc.1998.8243
发表时间：
1998-03-06
影响因子：
3.1
作者：
Ide, N;Hata, Y;Takai, Y
通讯作者：
Takai, Y